猪肠道钠氢交换载体(NHE3)mRNA表达的肠段特异性和发育性变化  

Segmental Distribution and Ontogenetic Regulation of NHE3 mRNA Expression in Intestine of Pigs

作　　者：邹仕庚[1,2] 冯定远[1] 黄志毅[1] 李岩 吴同山[3] 左建军[1] 职爱民[1] 董泽敏[1] 张常明[1] 刘清神[1] 

机构地区：[1]华南农业大学动物科学学院,广州510642 [2]广东科贸职业学院,广州510430 [3]东莞市畜牧科学研究所,东莞523086

出　　处：《畜牧兽医学报》2009年第3期343-348,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重点基础研究发展计划(973)项目(2004CB117501)

摘　　要：旨在探讨猪肠道钠氢交换载体(NHE3)mRNA表达的肠段特异性和发育模式,为NHE在养猪生产中的应用提供理论依据。选取遗传背景相同的1、7、26、30、60、90和150 d蓝塘和长白公猪各5头,测体质量后屠宰,取十二指肠、空肠、回肠和结肠组织样品;以18S rRNA为内标基因,用实时荧光定量PCR法检测NHE3 mRNA在60 d长白猪表达的肠段特异性及其在蓝塘和长白猪肠道表达的发育模式。结果显示:长白猪肠道NHE3 mRNA的表达丰度为结肠、十二指肠、空肠和回肠依次降低,且结肠显著高于空肠和回肠(P<0.05)。不同猪种NHE3mRNA在十二指肠和空肠的表达模式相似;蓝塘和长白猪NHE3 mRNA的表达丰度分别在7和30 d(十二指肠)、7和26 d(空肠)达最高水平(P<0.05)。不同猪种结肠NHE3 mRNA的表达模式不同,分别与其在十二指肠和空肠的发育呈现不同的模式;蓝塘猪结肠NHE3 mRNA的表达丰度在26、90和150 d时显著低于长白猪(P<0.05)。以上结果说明,猪肠道NHE3 mRNA的表达受到发育阶段、品种和肠段的调控,且在十二指肠和空肠间具有品种稳定性。Segmental distribution and ontogenetic regulation of sodium hydrogen exchanger （NHE3） mRNA expression were evaluated in pigs along the horizontal axis of the intestine. A total of 35 littermate purebred Lantang gilts and 35 littermate purebred Landrace gilts were divid- ed into seven groups at the ages of day 1, 7, 26, 30, 60, 90 and 150, respectively. Samples of in- testinal segments （duodenum, jejunum, ileum and colon） were collected. The NHE3 mRNA a- bundance was determined by real-time RT-PCR using SYBR Green IRT-PCR mix Kit. Results showed that the abundance of NHE3 mRNA was highest in colon and lowest in jejunum and ileum （P〈0.05）. The ontogenetic expression of NHE3 mRNA in duodenum and jejunum of Lantang and Landrace pigs were similar. The highest expression abundance of NHE3 mRNA were 7 d of Lantang and 30 d of Landrace in duodenum, 7 d of Lantang and 26 d of Landrace in jejunum （P〈0. 05）. The expression pattern of NHE3 mRNA in colon was differed from duodenum and jejunum. Abundance of NHE3 mRNA of Lantang pigs on 26, 90 and 150 d were lower than that of Landrace pigs （P〈0.05） in colon. The results indicated that NHE3 mRNA expression could be regulated by developmental stages, breeds and segments of intestine in pigs.

关 键 词：猪 肠道 NHE3mRNA 肠段特异性 发育模式 

分 类 号：S828[农业科学—畜牧学] S852.2[农业科学—畜牧兽医]