pGRF基因质粒对免疫应激断奶仔猪生长抑制的缓解作用  

Alleviation Effect of pGRF Gene Plasmid on Growth Depression of Weaned Piglets after Immune Challenge

作　　者：董海军[1] 王康宁[1] 李霞[1] 

机构地区：[1]四川农业大学动物营养研究所四川省重点开放实验室,雅安625014

出　　处：《畜牧兽医学报》2009年第3期349-355,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：教育部长江学者和创新团队发展计划(IRT0555)资助

摘　　要：旨在研究pGRF基因质粒对免疫应激断奶仔猪生长的影响,探讨pGRF基因质粒缓解免疫应激引起的生长抑制作用及其可能的机理。选取18头日龄为(35±2)d、体质量为(7.86±0.59)kg未去势的杜洛克×长白×大白(DLY)三元杂交断奶仔猪,采用单向分类试验设计,按体质量相近、性别一致的原则配对,分为3个处理,即pGRF(pGRF基因质粒)组、pGRF+LPS(脂多糖)组和LPS组,每个处理6个重复,每个重复1头猪。结果表明:pGRF基因质粒可以缓解因注射LPS导致的日增质量的降低(P<0.05),饲料转化率有所改善(P>0.05);能够抑制由LPS诱导的细胞因子(IL-1I、L-6)浓度的上升(P<0.05或P<0.01)和血清IGF-I浓度的降低(P<0.05或P<0.01),同时显著提高血清IgG的浓度(P<0.05)。结论:pGRF基因质粒缓解了断奶仔猪因注射脂多糖引起的生长抑制,降低了血清IL-1和IL-6的水平,提高了血清IgG的浓度,提高了仔猪的抗病力,从而缓解了断奶仔猪的免疫应激;pGRF基因质粒缓解免疫应激的作用与其促进IGF-I的分泌密切相关。The purpose of this study was to evaluate the effect of pGRF gene plasmid on growth performance and immunological function of immune-challenged weaned piglets, and also the potential mechanism of these effects were discussed. Eighteen weaned piglets（Duroc× Landrace× Large White） with ages of （35±2）d and initial body weight （BW） （7.86±0.59）kg were not castrated and randomly assigned into three treatments according to gender and BW by single factor design. Three treatments were pGRF gene plasmid group, pGRF gene plasmid plus lipopolysac- charide（LPS） group and LPS group. Each treatment had six replicates and one piglet in each replicate. The results were as follows：The piglets in the pGRF gene plasmid plus LPS group had a better growth performance than those in the LPS group（P〈0.05）. The F/G of piglets in the pGRF gene plasmid plus LPS group was lower than that in the LPS group（P〈0. 05）. The serum IGF-1 level in the pGRF gene plasmid plus LPS group was significantly higher than that in the LPS group（P〈0.05 or P〈0.01）. The serum IgG level in the pGRF gene plasmid plus LPS group was higher than that in the LPS group（P〈0.05）. The serum IL-1 and IL-6 levels in the pGRF gene plasmid plus LPS group were significantly lower than that in the LPS group（P〈0.05 or P〈0.01）. In conclusion, compared with the LPS group, in the pGRF gene plasmid plus LPS group had lower levels of serum IL-1 and IL-6 and higher levels of serum IgG. The statuses of immune challenge induced by LPS injection was improved by pGRF gene plasmid. The characteristics of resisting diseases were increased. However, the characteristics of pGRF＇s immunoregulation may closely related to the secretion of IGF-I.

关 键 词：PGRF基因质粒 免疫应激 断奶仔猪 生产性能 免疫功能 

分 类 号：S828.5[农业科学—畜牧学]