中国水禽新城疫病毒F和HN基因的变异趋势  

Tendency of Genetic Variation on the F and HN Genes of Newcastle Disease Viruses Isolated from Waterfowls in China

作　　者：徐怀英[1] 秦卓明[1] 王友令[1] 欧阳文军[1] 谭雷涛[1] 何叶峰[1] 金维江[1] 袁小远[1] 

机构地区：[1]山东农业科学院家禽研究所,济南250100

出　　处：《畜牧兽医学报》2009年第3期394-401,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：山东省科技攻关重点支持项目(030317);山东省农科院创新基金项目(2006YCX024)

摘　　要：选取中国1996-2005年的8株鹅、鸭等水禽NDV流行株,克隆其融合蛋白(F)和血凝素-神经氨酸酶(HN)基因,参考GenBank中具有F和HN核苷酸全长的16株国内外NDV代表株、16株国内水禽流行株以及12株只具有F基因的水禽毒株序列,参照传统F基因分型方法,分别对F和HN基因进行分型,同时进行相关比较、核苷酸和氨基酸同源性以及系统发育分析。结果表明:16株国内外代表株F和HN基因分型基本一致,但24株水禽NDV流行株中只有15株相同,符合率为62.5%,不同占37.5%(9/24)。系统发育和同源比较发现:水禽NDV流行株HN基因高度同源,同属Ⅶ型,而F基因则分属不同的基因型。通过对24株NDVF和HN基因氨基酸全长以及进行F基因分型的F基因47-420位核苷酸的同源性相关性比较证实:F基因前47-420位核苷酸的遗传变异与F基因氨基酸全长变异密切相关,r=0.916,但与HN基因的氨基酸变异不相关,显示出NDV在演变过程中F和HN基因具有相对的独立性。水禽NDV是家禽NDV的重要储存宿主,应高度重视水禽NDV的变异。Eight Newcastle disease virus （NDV） isolates from waterfowl such as geese and duck were obtained in China during 1996-2005, and then the fusion （F） protein gene and haemaggluti- nin-neuraminidase （HN） gene of those isolates were amplified and sequenced for further phyloge- netic analysis. Phylogenetic trees were constructed separately based on variable region fragment （47-420 nt） of the F gene and full-length deduced amino acid sequence of the HN gene of 40 NDV strains, including 16 NDV reference strains in the world and the 24 prevalent waterfowl NDV iso- lates. The results showed that 16 NDV reference strains were identical in the two different phylo- genetic trees. While, only 15 in 24 NDV isolates from waterfowl were similar, the others were different. In addition, 24 waterfowl-derived NDV strains were all classified as genotype Ⅶ on the basis of deduced amino acid sequence of the HN gene, which were different from that by F gene. Furthermore, there had feeble correlation between the complete F and HN protein sequences, and highly close correlation between full-length F protein sequences and variable region fragments. The results showed that evolutionary rates of the F and HN genes were respectively independent. All those data showed that waterfowls might be the major reservoir hosts of NDV and we should pay more attention to those NDV strains isolated from waterfowls.

关 键 词：新城疫病毒 F基因 HN基因 同源性 遗传变异 相关性 

分 类 号：S852.659.5[农业科学—基础兽医学]