I型胶原和热休克蛋白90在肉鸡胫骨软骨发育不良不同阶段的变化  

Changes of Collogen Type I and Heat Shock Protein 90 at the Different Stages of Broiler Tibial Dyschondroplasia

作　　者：田文霞[1,2] 覃平[1] 张艳红[1] 李家奎[1] 毕丁仁[1] 潘思轶[3] 郭定宗[1] 

机构地区：[1]华中农业大学动物医学院,武汉430070 [2]山西农业大学动物科技学院,太谷030801 [3]华中农业大学食品科技学院,武汉430070

出　　处：《畜牧兽医学报》2009年第3期428-432,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30571370)

摘　　要：应用免疫组织化学方法,研究了I型胶原(Col I)和热休克蛋白90(Hsp90)在肉鸡胫骨软骨发育不良(TD)不同阶段的变化。将80羽1日龄健康AA肉鸡预饲1周后分为2组。对照组饲以基础日粮;试验组在基础日粮中添加福美双100 mg.kg-1,4 d后继续饲以基础日粮。试验历时23 d。应用抗Col I和Hsp90多克隆抗体对试验第4、9、16、23天的肉鸡胫骨生长板进行免疫组织化学研究。结果表明,在饲喂福美双后第4、第9天,Col I和Hsp90的合成在生长板前肥大区和肥大区软骨细胞都明显增加,而第16、第23天时在TD损伤周边的前肥大区软骨细胞出现。由此推测,Col I蛋白合成增加,可能是由于不能正常钙化的反馈调节所致;而上调表达的Hsp90可能在调节软骨细胞发育周期中发挥重要作用,为进一步认识TD提供了新的思路。The experiment was conducted to identify the changes of collogen type I （Col I） and heat shock protein 90 （Hsp90） at the different stages of broiler tibial dyschondroplasia. 80 one day-old AA broiler chickens were randomly divided into two groups after being raised seven days. The control group was given a regular diet, whereas the experimental group was given the same diet containing thiram 100 mg ·kg ^-1 for four days, then both of the groups was given the same regular diet. The feeding experiment lasted 23 days. Immunohistochemistry was adopted to detect protein synthesis of Col I and Hsp90 in tibia growth plate at the fourth day, the ninth day, the sixteenth day, the twenty-third day respectively by using Col I and Hsp 90 polyclonal antibody. The results showed that synthesis of Col I and Hsp90 was elevated remarkably in prehy- pertrophie and hypertrophic chondroeytes at the fourth day and the ninth day after being fed thiram diet, but the synthesis of Col I and Hsp90 was identified in prehypertrophie chondrocytes a round TD lesion at the sixteenth day and the twenty-third day respectively. It was the non calcification feedback regulation might have resulted in upregulated Col I in deduced that TD and the increased Hsp90 might play an important role in regulating cartilage cell cycle. As a result, the research provides new insights into understanding the pathogenic process of TD in chickens.

关 键 词：福美双 肉鸡 胫骨软骨发育不良 I型胶原(ColI) 热休克蛋白90(Hsp90) 

分 类 号：S856[农业科学—临床兽医学]