高致病性猪蓝耳病病毒GS/LZh/07株的分离、鉴定及其非结构蛋白Nsp2基因特性分析  

Isolation,Identification and Characteristic Analysis of Non-structural Protein Nsp2 Gene of High Pathogenicity Porcine Reproductive and Respiratory Syndrome GS/LZh/07 Strain

作　　者：吴锦艳[1] 田宏[1] 尚佑军[1] 刘湘涛[1] 郑海学[1] 靳野[1] 尹双辉[1] 满自萍[1] 赵娜[1] 蔡承茹[1] 蔺芳[1] 谢庆阁[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点开放实验室,兰州730046

出　　处：《畜牧兽医学报》2009年第3期438-443,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家863高技术研究发展计划(863)资助项目(2006AA241110)

摘　　要：将高致病性猪蓝耳病疑似病料接种Marc145细胞,发现该病料可使Marc145细胞产生CPE,应用猪蓝耳病病原检测试剂盒从细胞培养物中检测到PRRSV,命名为Gs/Lzh/07株。同时应用RT-PCR方法扩增该分离毒株的非结构蛋白Nsp2基因,并进行了序列测定,发现所扩增到的Nsp2基因有90个核苷酸的缺失。序列比对结果表明:该分离病毒Nsp2基因与经典毒株PRRSV-ch-1a核苷酸同源性为83.0%,氨基酸同源性为72.7%;与高致病性变异毒株NX和SD核苷酸同源性为95.5%,氨基酸同源性为90.9%,可见该毒来源于2006-2007年流行毒株,说明高致病性猪蓝耳病变异病毒已经在甘肃省存在。该毒株的成功分离为高致病性猪蓝耳病流行病学调查分析提供数据,也为预防控制该病积累了资料。Nsp2的特性分析为揭示高致病性猪蓝耳病PRRSV在甘肃省的流行特点提供参考。PRRS suspect patho-material was inoculated in Marc145 and CPE was found, a strain of PRRSV was detected from cell cultures by PRRSV kit and named as GS/LZh/07. Meanwhile non-structural protein Nsp2 gene was obtained by RT-PCR,Nsp2 90 nucleotide deletion in Nsp2 gene was detected by sequencing. Sequence analysis indicated that homology between GS/LZh/07 and classical strain was 83.0% in nueleotide sequence and 72.7% in amino acids sequence; homology between GS/LZh/07 and HP-PRRSV variation NX and SD was 95.5% in nucleotide sequence, and 90.9% in amino acids sequence. It was indicated that GS/ LZh/07 originated from popular strain 2006-2007. The results illustrated that HP-PRRSV variation strain exists in Gansu province. Succeed isolation of GS/LZh/07 can provide data for epidemiology of HP-PRRSV, at the same time it can accumulate document for preservation and manipulation of the disease, char- acteristic analysis of Nsp2 may offer information for HP-PRRSV popular feature in Gansu province.

关 键 词：高致病性猪蓝耳病病毒 分离 鉴定 NSP2 特性分析 

分 类 号：S852.659.6[农业科学—基础兽医学]