双歧杆菌脂磷壁酸延缓细胞衰老的作用机制  

Effect and mechanism of lipoteichoic acid of Bifidobacterium on delaying cell aing

作　　者：彭艳华[1] 王跃[2] 刘健龙[1] 刘明方[1] 陈淑惠[1] 

机构地区：[1]重庆医科大学医学检验系临床检验诊断学省部共建教育部重点实验室,重庆400016 [2]成都医学院医学检验系

出　　处：《中国老年学杂志》2009年第6期668-670,共3页Chinese Journal of Gerontology

摘　　要：目的探讨双歧杆菌脂磷壁酸(Lipoteichoic acid,LTA)在延缓H2O2诱导细胞衰老中的作用。方法H2O2诱导WI-38细胞衰老,β-半乳糖苷酶细胞化学染色计算衰老细胞百分率变化,RT-PCR和Western印迹检测衰老细胞p21、细胞周期蛋白E(cyclin E)和周期蛋白依赖性蛋白激酶2(CDK2)表达水平的变化。结果双歧杆菌LTA处理后,β-半乳糖苷酶细胞化学染色阳性细胞百分率较衰老模型组降低(P<0.01)。与年轻对照组相比,衰老模型组细胞中p21的表达增高,cyclin E和CDK2表达降低,而双歧杆菌LTA能够逆转上述变化(P<0.01)。结论双歧杆菌能延缓H2O2诱导的细胞衰老,机制可能与改变p21,cyclin E和CDK2表达水平有关。Objective To explore the effect of lipoteichoic acid （LTA） of Bifidobacterium on delaying cell aging induced by H2O2. Methods WI-38 cell aging was induced by H2O2.β-galactosidase （β-gal） cytochemistry staining was used to evaluate the percent of aging cell and the mRNA and protein levels of p21, cyclin E and CDK2 were detected by RT-PCR and Western blot. Results Treatment with LTA（5, 10 μg/ml）led to a significant increase in the proportion of β-gal-positive ceils （P 〈 0. 01 ） and markedly decreased the mRNA and protein levels of p21 and increased the mRNA and protein levels of cyclin E and CDK2 in WI-38 cells in aging model group （all P 〈0. 01 ）. Conclusions LTA of Bifidobacterium can delay cell aging induced by H2O2, whose mechanism may have relation with change the expressions of p21, cyclin E and CDK2.

关 键 词：双歧杆菌 脂磷壁酸 细胞衰老 H2O2 

分 类 号：R339.38[医药卫生—人体生理学]