包裹光敏剂m-THPC的PLGA微球的体外释放特性研究  

The Release Study in Vitro of PLGA Microparticles Enwrapping Photosensitizer m-THPC

作　　者：汪峰[1,2] 任山[1] 赵箭光[2] 刘向阳[1] 

机构地区：[1]中山大学纳米技术研究中心,广东广州510275 [2]广州医学院生物医学工程系,广东广州510182

出　　处：《中国医学物理学杂志》2009年第2期1120-1123,共4页Chinese Journal of Medical Physics

摘　　要：目的:测量包裹光敏剂m-THPC的PLGA微球的载药量,并定量分析在含不同浓度脂肪乳和血清的水溶液中PLGA微球的体外释放规律。方法:称取一定质量PLGA微球,置入一定体积的二氯甲烷和甲醇混合有机溶剂中完全溶解,测量溶液荧光强度,与荧光强度—光敏剂m-THPC浓度标准曲线对比得出光敏剂m-THPC的浓度后计算出PLGA微球的载药量;称取一定质量的载药量已知的PLGA微球,置入200mL含脂肪乳和血清的水溶液中释放,37℃恒温摇床摇动,每次取样0.5mL,测量溶液荧光强度,与荧光强度—光敏剂m-THPC浓度标准曲线对比得出光敏剂m-THPC的浓度后计算出光敏剂m-THPC的释放量。结果:血清能加速PLGA微球的释放,同时脂肪乳浓度越高,PLGA微球释放光敏剂m-THPC的速度越快。结论:给病人注射适量的脂肪乳注射液,有可能提高PLGA微球释放速度和光敏剂m-THPC在血液中的浓度,从而提高光动力治疗效率。Objective： Determination of the drug loading of the PLGA microparticles enwrapping photosensitizer m-THPC ,and quantitative analysis of the m-THPC release in vitro of these PLGA microparticles in water solution containing intralipid and serum. Methods： Dissolving some PLGA microparticles in fl-e mixture solution of Methylene Dichloride and Methyl Alcohol, and the concentration of m-THPC in the solution is determined by comparing the fluorescence intensity of the solution with the prefabricated standard intensity-concentration curve, thus the drug loading of the PLGA microparticles can be calculated. Dissolving PLGA micropartMes whose drug loading is known in 200 mL water solution of intralipid and serum, and stir the solution at 37℃. Taking 0.5 mL sample each time fiom the solution, and the concentration of m-THPC is determined by comparing the fluorescence intensity of the sample with the prefabricated standard intensity-concentration curve, so the quantity of the m-THPC released from these PLGA microparticles can be calculated. Results： The m-THPC release rate of these PLGA microparticles can be accelerated by increasing the concentration of intralipid or serum. Conclusion： The m-THPC release rate of these PLGA microparticles in the blood of the patient can be accelerated if the patient has been injected with some intralipid. This will improve the concentration of m-TttPC in the blood as well as the efficiency of the photo dynamic therapy on the patient.

关 键 词：光动力治疗 光敏剂 乳化法 体外释放 

分 类 号：R454.2[医药卫生—治疗学]