低剂量γ射线辐照外周血单个核细胞对细胞因子表达的影响  

Effect of Low Dose Gamma Ray Radiation on the Express of Cytokines in Peripheral Blood Monounclear Cells

作　　者：张献清[1,2] 穆士杰[2] 夏爱军[2] 易静[2] 安群星[2] 陈蕤[2] 吴道澄[1] 

机构地区：[1]西安交通大学生物医学信息工程教育部重点实验室,陕西西安710038 [2]第四军医大学西京医院输血科,陕西西安710032

出　　处：《中国医学物理学杂志》2009年第2期1124-1125,1146,共3页Chinese Journal of Medical Physics

摘　　要：目的:探讨低剂量辐射对外周血单个核细胞细胞因子表达的刺激效应。方法:人外周血单个核细胞采用1Gyγ射线照射,吸收剂量率为17Gy/min,并在培养过程中不同时间采用ELISA方法检测上清液中IL-2、TNFα及IFN-γ含量。结果:经γ射线照射24h后培养上清中IL-2、TNFα及IFN-γ含量明显升高,与对照组比较有明显差异(P<0.05),并随培养时间延长,各细胞因子含量会进一步升高。结论:低剂量辐射对外周血单个核细胞中IL-2、TNFα及IFN-γ表达具有刺激效应。Objective： To investigate the hormesis of low dose radiation on the expression of cytokines in peripheral blood mononuclear cells （PBMCs）. Methods： Human PBMCs were irradiated by gamma ray at 1 gray. The absorption dose rate was 17 Gy/min. Contents of interleukin-2（IL-2）,tumor necrosing factor-α（TNFα） and interferon-γ（IFN-γ） were detected by ELISA in culture supemates of PBMCs at different period. Results： Twenty-four hours after radition, the contents of IL-2,TNF α and IFN-γ in culture supemates were obviously increased and have obvious difference with that of control group （P〈0.05）. Along with the extend of culture time, the contents of cytokines more increased. Conclusions： Low dose radiation with gamma rays can induce hormesis as to the expression of IL-2 、TNFα and IFN-γin PBMCs.

关 键 词：外周血单个核细胞 Γ射线 低剂量辐射 细胞因子 

分 类 号：R331.142[医药卫生—人体生理学] R811.5[医药卫生—基础医学]