13株中国麻疹野病毒血溶素蛋白基因特征分析  

The Genetic Characteriistics Analysis of Fusion Gene of 13 Strains Measles Viruses in China

作　　者：许松涛[1] 吴宏伟[2] 朱贞[1] 张燕[1] 崔爱利[1] 毛乃颖[1] 蒋小泓[1] 郑焕英[3] 周淑洁[4] 玛合木提[5] 赵春芳[6] 郑蕾[7] 张红[8] 王艳[9] 王建国[10] 张菊英[11] 刘杨[12] 林春燕[13] 司源[14] 许文波[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,病毒基因工程国家重点实验室,北京100050 [2]北华大学附属医院,吉林吉林132000 [3]广东省疾病预防控制中心,广州510300 [4]安徽省疾病预防控制中心,合肥230061 [5]新疆维吾尔自治区疾病预防控制中心,乌鲁木齐830011 [6]重庆市疾病预防控制中心,重庆400042 [7]山西省疾病预防控制中心,太原030012 [8]湖南省疾病预防控制中心,长沙410005 [9]辽宁省疾病预防控制中心,沈阳110005 [10]上海市疾病预防控制中心,上海200336 [11]河南省疾病预防控制中心,郑州450000 [12]天津市疾病预防控制中心,天津300011 [13]海南省疾病预防控制中心,海口570203 [14]陕西省疾病预防控制中心,西安710054

出　　处：《中国疫苗和免疫》2009年第1期1-7,共7页Chinese Journal of Vaccines and Immunization

基　　金：卫生部疾病预防控制专项2008;世界卫星组织项目(编号:I8/181/978)

摘　　要：目的分析1999～2003年中国(未包括香港、澳门特别行政区和台湾地区,下同)流行的H1基因型麻疹野病毒代表株血溶素蛋白(Fusion Protein,F)基因的分子特征。方法从1999～2003年中国分离的麻疹野病毒株中,选取13株H1基因型代表株,包括H1a基因亚型麻疹野病毒8株和H1b基因亚型麻疹野病毒5株。使用逆转录-聚合酶链反应扩增病毒的F基因全长,并进行核苷酸序列测定,同时与中国疫苗株及其它国家的A、D3、D6、D7、E基因型参考株进行序列比对及基因亲缘性关系分析。结果1999～2003年13株中国流行麻疹野病毒代表株中,核苷酸同源性为98.1%～99.0%,氨基酸同源性为98.1%～100.0%,与中国疫苗株沪191相比,其核苷酸同源性为95.7%～96.2%,氨基酸同源性为96.9%～97.2%;而与其它基因型相比,核苷酸同源性为94.4%～96.2%。F基因3个糖基化位点(第32、64、70位氨基酸)、对病毒融合功能起着重要作用的第112位精氨酸、第195位亮氨酸未发生改变。结论1999～2003年中国流行的H1基因型麻疹野病毒的F基因无明显差异,F蛋白上已知的重要功能位点未发生变异,推测中国流行的麻疹野病毒F蛋白的结构和功能未发生较大改变,但由于F蛋白是重要的功能蛋白,对F蛋白核苷酸和氨基酸的变异规律进行常规监测,对了解麻疹野病毒流行特点及其遗传变异规律具有重要意义。Objective To study genetic characteristics of fusion gene （F gene） of H1 genotype of wild measles virus circulated in China from 1999 to 2003. Method 13 H1 genotype representative strains including 8 subgenotype Hla strains and 5 subgenotype H1b strains isolated in China's Mainland during 1999 -2003 were selected. Entire F gene were amplified by reverse transcript-polymerase chain reaction and sequenced, then they were compared with the nucleotide acid sequences of Chinese measles vaccine strains and the representative strains of A,Da ,D6 ,D7 and E genotypes from other countries. The phylogenetic analysis were conducted. Results Among 13 representative strains circulated in China during 1999 to 2003,the homology of nucleotide acid and amino acid were 98. 1%499.0% and 98. 1%～100% respectively,and were 95. 7% ～ 96.2% and 96. 9% ～97. 2% respectively compared with Chinese vaccine strains,while compared with other genotype strains, the homology of nucleotide acid were 94.4% 96.2%. In F gene,the 3 glycosylation site （aa32,aa64 and aa70）, aa112 and aa195 which played the impor- tant role to viral fusion had no changes. Conclusion The F genes of H1 genotype measles virus circulated in china mainland during 1999-2003 had no significant vibration, and known important functional sites in Fusion protein didn＇t have deviation,it could be postulated that the structure and functions of F protein of wild Measles viruses circulated in China's Mainland from 1999 to 2003 were conserved. F protein,however,played an important role in Measles viruses,it need to conducted routine monitoring to F gene. which benefit for understanding of epidemic features and genetic variation pattern of wild Measles viruses.

关 键 词：麻疹病毒 血溶素蛋白 基因特征 

分 类 号：R373.11[医药卫生—病原生物学]