中国2006～2008年流行性腮腺炎病毒的基因特征分析  

Genetic Characteristics of Mumps Virus in China From 2006 to 2008

作　　者：崔爱利[1] 朱贞[1] 王常银[2] 王艳[3] 周剑惠[4] 吴宏伟[5] 王爽[4] 常新[4] 檀晓娟[1] 张燕[1] 毛乃颖[1] 许文波[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,病毒基因工程国家重点实验室,北京100050 [2]山东省疾病预防控制中心,济南250014 [3]辽宁省疾病预防控制中心,沈阳110005 [4]吉林省疾病预防控制中心,长春130062 [5]北华大学附属医院,吉林吉林132000

出　　处：《中国疫苗和免疫》2009年第1期8-13,共6页Chinese Journal of Vaccines and Immunization

基　　金：卫生部疾病预防控制专项(2006~2008年)

摘　　要：目的分析中国(未包括香港、澳门特别行政区和台湾地区,下同)2006～2008年流行性腮腺炎(腮腺炎)病毒(Mumps Virus,MuV)的基因特征。方法针对中国2007～2008年分离到的7株MuV小疏水蛋白(Small Hydro-phobic Protein,SH)基因316个核苷酸片段进行逆转录-聚合酶链反应(Polymerase Chain Reaction,PCR)扩增,并对该PCR产物进行序列测定,结合从基因库下载的7株2006年中国MuV毒株序列以及世界卫生组织(WHO)MuV基因型参考株一起进行分子流行病学研究。结果通过比较核苷酸和氨基酸同源性和构建亲缘关系树发现,中国2006～2008年14株MuV分离株同属F基因型,但序列相互间存在差异。不同省份、不同年代的MuV毒株在亲缘关系树上呈分散交叉分布,无明显的时间和地理分布倾向。此外,通过分析2006～2008年MuV的组内和组间遗传距离发现,2006～2008年的MuV毒株序列无明显规律性变化。与序列同源性和亲缘关系树分析结果一致。结论中国2006～2008年流行的MuV同属F基因型,是由F基因型MuV的多个传播链引起的。并且1995年和2006～2008年的MuV毒株间序列差异较大,说明1995～2008年中国流行的MuV发生了一定程度的变异。另外,还发现F基因型MuV在SH基因上存在着特异性突变(CNt65、CNt105、GNt137、CNt192、CNt239),而其它基因型MuV在这些位点上均未发生改变。Objective To analyze genetic characteristics of mumps virus in china from 2006 to 2008 Methods In the study, the fragments containing 316 nucleotides of SH genes from 7 mumps virus isolates in China from 2007 to 2008 were amplified by RT-PCR method, the PCR products were directly sequenced and analyzed with other 7 mumps virus sequences in China in 2006 and WHO mumps reference strains downloaded from Genbank together. Results The re- sults of homology and phylogenetic analyses based on 316 nucleotides showed that all 14 Chinese mumps virus strains in 2006--2008 belong to a distinguish branch of F genotype,but there were some differences between these 14 mumps virus strains. The mumps virus sequences in different provinces or different years showed dispersed distributions in phylogenetic tree,which was no ob- vious tendency of the distribution for years and geography. Otherwise, with the analyses of genetic distance between and within mumps virus strains groups in 2006,2007 and 2008, it was found that the mumps sequences in 2006--2008 were no obvious regular variation, which were similar with the results of homology and phylogenetic analyses. Conclusion It suggested that F genotype of mumps virus was the predominant genotype in 2006- 2008, and the mumps prevailed in recent years was mainly caused by F genotype mumps viruses with multi-transmission routes. However, the mumps viruses circulated during 2006 to 2008 were different from those in 1995 ,these mumps virus occured genetics evlolution in some extent during 14-year period from 1995 to 2008. Furthermore,comparing with other genotype mumps strains from other countries, there were 5 shared nucleotides mutations（CNt65 , CNtl05 , GNt137 , CNt192 , CNt239 ） in SH gene in all Chinese F genotype mumps viruses. However, these mutations were not found in the other genotype of mumps virus.

关 键 词：流行性腮腺炎病毒 基因特征 基因型 

分 类 号：R373.16[医药卫生—病原生物学]