毛细管电泳非接触电导检测器的研制(英文)  

Contactless Conductivity Detector for Capillary Electrophoresis Chip

作　　者：刘晓为[1,2] 张海峰[1] 邵宪辉[1] 李玲[1] 彭志成[1] 

机构地区：[1]哈尔滨工业大学MEMS中心,哈尔滨150001 [2]微系统与微结构制造教育部重点实验室,哈尔滨150001

出　　处：《纳米技术与精密工程》2009年第2期123-126,共4页Nanotechnology and Precision Engineering

摘　　要：非接触电导检测法已经成为毛细管电泳芯片中一种通用的物质分离检测方法.本文提出了一种微型化的电容耦合非接触电导分析系统,采用新颖的三明治电极结构减小了电极之间的寄生电容.这种电极结构能够有效地减小电极的长度,克服了非接触电导检测中电极易于折断的缺点.当采用电极宽度1,mm、电极间距1,mm、MES/His缓冲溶液浓度为20,mmol/L和激励电压20,Vpp、90,kHz的条件时,检测器具有最好的分离效果.在最佳的分离条件下,实现了1mmol/L的K+和Mg2+混合无机阳离子的分离检测.Contactless conductivity detection has become an accepted detection method in capillary electrophoresis chip for a variety of analytes. In this paper, a miniaturized analytical system with capacitively coupled contactless conductivity detection was presented. Polymethyl methacrylate （PMMA） was used as substrate to substitute for glass material. A novel sandwich electrode structure was used to minimize the stray capacitance of the detector. The construction can decrease the length of the electrode and overcomes the disadvantage of electrode frangibility. Experimental parameters relevant to sepa- ration and detection processes were optimized to separate the mixture of inorganic cations K＋ and Mg2＋ （1 mmol/L） with electrodes of 1 mm width, 20 mmol/L MES/His buffer solution and a detection gap of 1 mm when operating the detector at 20 Vpp and 90 kHz.

关 键 词：毛细管电泳 非接触电导检测法 PMMA 电极结构 无机阳离子 

分 类 号：TH832[机械工程—仪器科学与技术]