利用高代回交和分子标记辅助选择构建棉花染色体片段代换系  

Developing Chromosome Segment Substitution Lines (CSSLs) in Cotton (Gossypium) Using Advanced Backcross and MAS

作　　者：杨泽茂[1,2] 李骏智[1,2] 李爱国[2] 张保才[2] 刘广平[2] 李俊文[2] 石玉真[2] 刘爱英[2] 蒋建雄[1] 王涛[2] 袁有禄[2] 

机构地区：[1]湖南农业大学生物科学技术学院,长沙410128 [2]中国农业科学院棉花研究所,农业部棉花遗传改良重点实验室,安阳455000

出　　处：《分子植物育种》2009年第2期233-241,共9页Molecular Plant Breeding

基　　金：国家973计划项目(2004CB117306);中央级公益性科研院所基本科研业务专项(SJA0801)资助

摘　　要：染色体片段代换系(chromosome segment substitution lines,CSSLs)又叫导入系(introgression lines,ILs),是在相同的遗传背景中导入供体亲本的染色体片段,如果代换系中只含有一个来自供体亲本的染色体片段则称为单片段代换系(single segment substitution lines,SSSLs)。本实验利用陆地棉栽培种CCRI221(中棉所45)为受体,海岛棉海1为供体,通过高代回交和分子标记辅助选择相结合的方法,构建了1个由116个家系组成的染色体片段代换系群体。利用分布在棉花基因组25个连锁群上的276个多态性标记对BC4F1世代进行检测。这些标记覆盖了棉花基因组2347cM,占棉花基因组4450cM的52.7%。在每个家系中,代换片段最多的55个,最少的15个,平均32.92个,覆盖棉花基因组180.7～969cM的,平均覆盖了502cM。占检测长度(2347cM)的21.4%。在各家系中,每个连锁群平均包含0.1～8.3个海岛棉片段,平均覆盖每个连锁群0.2～54.2cM,占每条连锁群被检测长度0.5～207cM的10.3%～35.5%。本研究为棉花染色体单片段代换系的创制奠定了基础。Chromosome segment substitution lines （CSSLs）, also called as introgession lines （ILs）, carry a single or fewer donor segments in the same background of a recurrent genotype. If only a single donor segment, they are called single segment substitution sines （CSSLs）. In the study, a cotton population consisted of 116 CSSLs were developed from advanced backcrosses between the recipient, CCRI221, a cultivar of cotton, and donor hail, a barbadense cotton by microsatellite marker-assisted selection （MAS）. 116 BC4Ft lines were screened using 276 SSR primer pairs with polymorphism between ricipiend and donor in 25 linkage groups, which covered 2 347 cM, approximately 52.7% of cotton genome screened. There are 15 to 55 donor segments in lines with average of 32.92, which cover 180.7 cM to 969 cM of cotton genome. Average size of cotton genome covered by substitution segments are 502 cM, covered 21.4% of cotton genome detected. Average donor segments are 0.1-8.3 in each linkage group of in each BCnF1 line, which average size covered each linkage group ranges from 0.2 cM to 54.2 cM, 10.3-35.5% of the average long screened, ranged from 0.5 cM to 207 cM, of each linkage group in every lines.

关 键 词：棉花 染色体片段代换系 QTL 分子标记辅助选择 

分 类 号：S562[农业科学—作物学]