控制棉花长绒和短绒纤维生长发育的基因型分析  

Genetic Analysis on Genotypes of Cotton Fibrils of Lint and Fuzz

作　　者：叶磊[1] 石之光[2] 巩鹏涛[3] 李永起 方宣钧[3] 

机构地区：[1]广西大学生命科学与技术学院,南宁530005 [2]贵州大学生命科学学院,贵阳550025 [3]海南海德热带农业资源研究所,三亚572025

出　　处：《分子植物育种》2009年第2期242-250,共9页Molecular Plant Breeding

基　　金：国家支撑项目(2007BAD59B05)资助

摘　　要：本研究利用在纤维性状上有明显差异的4个棉花种质DP99B(毛籽棉,陆地棉)、FLS2123(裸籽棉,陆地棉)、ZYS20(光籽棉,陆地棉)以及VH8(光籽棉,海岛棉)作为棉花实验材料。利用以上4个棉花材料作为亲本组配了4个杂交组合,其组合分别为:DP99B(毛籽)×FLS123(裸籽)、DP99B(毛籽)×ZYS20(光籽)、ZYS20(光籽)×FLS123(裸籽)、DP99B(毛籽)×VH8(光籽),获得以上4种组合的F1代种子及F2代群体种子,分析F1、F2种子棉纤维性状,试图弄清控制棉花长短纤维分化和发育的基因型。根据本研究4种杂交组合其F1及F2分离表型结果,推定供试亲本材料的长绒生长发育的基因型可能为:DP99B(毛籽)的基因型为:LiALiA或LiAliA及LiDLiD或LiDliD;ZYS20(光籽)的基因型为:LiALiA或LiAliA及LiDliD或LiDliD;FLS2132(裸籽)的基因型为:LiALiALiDliD;VH8(光籽)的基因型为LiALiA或LiAliA及LiDLiD或LiDliD。4个组合的长绒的长度上都成较好的正态分布,认为棉花长纤维长度的发育是一种数量性状遗传。就短绒而言,在4个组合的F2代群体里的,ZYS20×FLS2132全为光籽、DP99B×VH8全为毛籽没有出现分离,DP99B×FLS2132和DP99B×ZYS20出现了分离,分析以上分离的具体情况并结合以前研究者结论,可以推断控制短绒和长绒分化和发育的基因并非同一组基因。推定出4个亲本DP99B(毛籽棉)、FLS2123(裸籽棉)、ZYS20(光籽棉)、VH8(光籽棉)控制短绒发育的几种可能的基因型。DP99B可能的基因型为:ii,susu,Ft1Ft1,Ft2Ft2,Ft3Ft3,FcFc;ii,susu,Ft1Ft1,Ft2ft2,Ft3ft3,Fcfc;VH8可能的基因型为:Ii,Susu,Ft1Ft1,Ft2Ft2,Ft3Ft3,FcFc;Ii,susu,Ft1Ft1,Ft2Ft2,Ft3Ft3,FcFc,ZYS20可能的基因型为:Ii,Susu,Ft1ft1,Ft2ft2,Ft3ft3,Fcfc;Ii,susu,Ft1ft1,Ft2ft2,Ft3ft3,Fcfc;ii,Susu,ft1ft1,ft2ft2,Ft3Ft3,fcfc;FLS2132可能的基因型为:II,SuSu,Ft1ft1,Ft2ft2,Ft3ft3,Fcfc;Ii,Susu,Ft1ft1,Ft2ft2,Ft3ft3,Fcfc。同时进一步推定出其4个组合DP99B×FLS123,DP99B×ZYS20,ZYS20×FLS123,DP99B×VH8其F1代控制短绒发育的基因In present study, the four cotton germplasms, Bollgard DP99B （full haired seed, Gossypium hirsutum L.）, ZYS20 （fuzzless seed, G. hirsutum L.）, FLS2123 （fuzzless-lintless seeds, G. hirsutum L.）, and VH8 （fuzzless seed, G. barbardense L.） were employed to be as parents for developing the geneticpopulations. We made four hybrid combinations that are DP99B×FLS 123, DP99B×ZYS20, ZYS20×FLS 123, and DP99B×VH8. In February, 2007, we harvested their F1 seeds of four combinations planted in the October of 2006, and F2 seeds harvested in the May of 2007. The fiber traits of F1 seed and F2, seed were phenotyped in order to make clear the genotypes of cotton fibrils of lint and fuzz. Based on the results of F1 fiber phenotypes and F2 fiber segregant phenotypes, it might deduct that the fibril genotypes of lint as follows： the genotype of the tested parent DP99B could be Li^ALi^A or Li^Ali^A and Li^DLi^D or Li^Dli^D; as well as ZYS20 be Li^ALi^A or Li^Ali^A and Li^DLi^D or Li^Dli^D; FLS2132 be li^Ali^A li^Dli^D; VH8 be Li^ALi^A or Li^Ali^A and Li^DLi^D or Li^Dli^D. The results indicated that cotton lint length showed a good normal distributions in all tested F2 populations, which implied that the development of cotton lint might be controlled by complex quantitative trait loci. Cotton fibril of fuzz in the F2 populations derived from the two combinations DP99B×ZYS20 and DP99B×VH8 were segregated in difference although their parent DP99B is full haired seed, ZYS20 and VH8 are both fuzzless seed and their FI seed are the full haired seeds. The former combination presented segregation, the latter combination without any segregation （all of full haired seed）. Cotton fuzz of the F2 population derived from DP99B × FLS2132 showed segregations but no segregation in the F2 population of ZSY20×FLS2132 （all of fuzzless seed）. These results suggested that the genes controlling the fibril differentiation of fuzz and lint be belonged to different genomes and the fibril genotypes of fuzz be assigned to be

关 键 词：棉花(Gossypium spp.) 毛籽棉 光籽棉 裸籽棉 长绒 短绒 基因型分析 

分 类 号：S562[农业科学—作物学]