水稻芽性状耐冷性的QTL分析  

QTL Analysis of Cold Tolerance for two Bud Traits in Rice

作　　者：巩迎军[1] 阮雯君[1] 荀星[1] 董彦君[1] 林冬枝[1] 叶胜海[2] 张小明[2] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234 [2]浙江省农业科学院,杭州310021

出　　处：《分子植物育种》2009年第2期273-278,共6页Molecular Plant Breeding

基　　金：国家航天育种工程项目(2006HT10001);上海市浦江人才计划(08PJ14085);上海市科委科技攻关计划项目(075405117);上海市教委重点项目(06ZZ21);上海市教委重点学科建设项目(J50401)共同资助

摘　　要：低温发芽和芽期耐冷性是影响水稻芽生长发育的两个重要因素。本试验利用182个越光(粳型)/Kasalath(籼型)//越光回交重组自交株系(backcross recombinant inbredlines,BILs),对2个芽性状耐冷性(低温发芽和芽期耐冷性)进行QTL(quantitative trait loci)定位和相关性分析。结果表明,BIL群体中这2个芽性状耐冷性均呈连续分布,属于数量性状遗传,两性状间的相关性不显著;控制低温发芽的4个QTL分布于第1、7、9、11染色体上,其贡献率为6.72%～12.78%;芽期耐冷性相关的4个QTL分布在第4、6(2个QTL)和11染色体上,贡献率为6.61%～14.93%;其中第11染色体上的2个QTL位于相同区域内,并且其增强耐冷性等位基因均来自耐冷性较差亲本Kasalath。本研究结果及其中检测到的QTLs两侧的连锁分子标记可为水稻芽性状耐冷性分子育种提供理论依据。Low-temperature germinability （LTG） and cold tolerance at bud-growth stage （CTS） are two important factors affecting the development of bud growth in rice. In this study, we located quantitative trait loci （QTL） for cold tolerance to two bud traits （LTG and CTS） and made correlation analysis by using a backcross inbred lines （BILs） derived from a backcross ofKoshihikari O＇aponica）/Kasalath （indica）//Koshihikari. Resultantly, continuous phenotypic variations for cold tolerance to the two bud traits studied were observed, indicating that they belong to quantitative trait inheritance. In addition, there wasn＇t significant correlation between them. Four QTLs for LTG were located on chromosome 1,7,9,11 and explained 6.72%-12.66% of the phenotypic variance, respectively; Four QTLs for CTS were located on chromosome 4, 6 （including 2 QTLs） and 11 with the contribution ratio range from 6.61% to 14.93%, respectively. Among them, the two QTLs （qLTG-11 and qCTS-11） on chromosome 11 were located in the same genomic region, and both alleles increasing cold tolerance of bud traits come from the Kasalath parent with weaker cold tolerance. These results and molecular markers tightly linked to QTLs detected in this study can provide useful information for molecular breeding of cold tolerance of bud traits in flee.

关 键 词：水稻 芽性状 耐冷性 QTL 

分 类 号：S511[农业科学—作物学]