冬小麦种质矮孟牛及其衍生后代1BL/1RS的分子和生化标记鉴定  

Identification of 1BL/1RS in Germplasm Aimengniu and its Derivatives via Biochemical and Molecular Markers

作　　者：赵春华[1] 崔法[1] 鲍印广[1] 宗浩[1] 王玉海[1] 王庆专[1] 王洪刚[1] 

机构地区：[1]山东农业大学作物生物学国家重点实验室,国家小麦改良中心泰安分中心,山东农业大学农学院,泰安271018

出　　处：《分子植物育种》2009年第2期307-311,共5页Molecular Plant Breeding

基　　金：国家973重大基础研究发展规划项目(2006CB101700)资助

摘　　要：本研究以小麦骨干亲本矮孟牛及其33个衍生品种(系)为材料,利用低分子量(LMW)麦谷蛋白Glu-B3位点的STS-PCR标记、醇溶蛋白Gli-B1位点的SSR标记和黑麦碱SEC-1b位点的STS-PCR标记进行复合PCR,检测1BL/1RS易位。结果表明,矮孟牛Ⅱ、Ⅳ、Ⅴ、Ⅵ和Ⅶ型含有1BL/1RS染色体,矮孟牛Ⅰ和Ⅲ型不含1BL/1RS;在矮孟牛的33个衍生后代中,25个含1BL/1RS,其余8个则不含1BL/1RS。利用A-PAGE技术对上述材料进行了黑麦碱蛋白的检测,结果与复合PCR一致,两种方法相结合能准确的检测1BL/1RS。GIu-B3 STS-PCR, GIi-B1 SSR and SEC-lb STS-PCR markers were used to identify 1BL/1RS translocation lines in Aimengniu and its derivatives. The results indicated that Aimengniu Ⅱ, Aimengniu Ⅳ, Aimengniu Ⅴ, AimengniuVI and Aimengniu Ⅶ were 1BL/1RS translocation lines, whereas Aimengniu Ⅰ and Aimengniu Ⅲ were not 1BL/1RS translocation lines. Among the 33 derivatives, there were 25 carrying 1BL/1RS translocation lines, while the others not. Secalins were detected by acid polyacrylamide gel electrophore- sis （A-PAGE）, and the results were accordant with that of multiplex PCR, so the combination of the two methods can test the 1BL/1RS translocation lines correctly.

关 键 词：冬小麦种质矮孟牛 生化和分子标记 1BL/1RS A—PAGE 复合PCR 

分 类 号：S512.1[农业科学—作物学] TQ325.15[化学工程—合成树脂塑料工业]