基于杂化材料和碳纳米管的硝酸还原酶电极  

Nitrate Reductase Electrode Based on Organic-Inorganic Hybrid Material and Carbon Nanotube

作　　者：闫博[1] 赵林[1,2] 谭欣[1,2] 

机构地区：[1]天津大学化工学院,天津300072 [2]天津大学环境科学与工程学院,天津300072

出　　处：《天津大学学报》2009年第3期253-257,共5页Journal of Tianjin University(Science and Technology)

基　　金：国家自然科学基金资助项目(20576092)

摘　　要：介绍了一种基于有机-无机杂化材料和碳纳米管的硝酸还原酶电极的制备方法.杂化材料由巯基乙酸接枝聚乙烯醇和二氧化硅溶胶组成,碳纳米管和硝酸还原酶同时包埋在杂化材料中,利用甲基紫精为电子媒介,制得新型的安培型硝酸还原酶电极.此酶电极可以在-0.70.V(vs SCE)下对硝酸根进行测定,碳纳米管的加入使响应电流增大了1倍左右.酶电极的线性范围为0.01～0.5.mmol/L,检测限为1.13×10-6.mol/L.平均响应时间为6.8 s左右.An enzyme electrode based on organic-inorganic hybrid material and carbon nanotube for nitrate detection was fabricated. The organic-inorganic hybrid material was composed of silica sol and a grafting copolymer of poly（vinyl alcohol）with thiogycolic acid. Nitrate reductase and carbon nanotubes were immobilized in the hybrid material. The nitrate reduction reaction was driven by the enzyme and methyl viologen which was used as the electron-transfer mediator. The electrode was applied to detect nitrate with operating potential of -0.70 V （vs SCE）. Carbon nanotube double enhanced the response current. The linearity range and detection limit were 0.01-0.5 mmol/L and 1.13 ×10^-6 mol/L. Average response time of the enzyme electrode was 6.8 s.

关 键 词：硝酸还原酶 酶电极 碳纳米管 有机-无机杂化材料 

分 类 号：O657.1[理学—分析化学]