清肝利湿汤干预慢性乙肝肝胆湿热证的蛋白质组学分析  

Proteomic analysis on chronic hepatitis B of liver-gallbladder dampness-heat type treated with Qinggan Lishi decoction

作　　者：戴姣[1] 戴幸平[1] 邢之华[1] 李萃[2] 李家邦[1] 蒋荣鑫[1] 

机构地区：[1]中南大学湘雅医院中西医研究结合所,湖南省长沙市410008 [2]中南大学湘雅医院卫生部肿瘤蛋白质组学重点实验室,湖南省长沙市410008

出　　处：《世界华人消化杂志》2009年第4期362-367,共6页World Chinese Journal of Digestology

基　　金：湖南省科技计划基金资助项目;No.06SK3028;中南大学理科发展基金资助项目;No.07SDF008~~

摘　　要：目的:分析清肝利湿汤治疗慢性乙肝(CHB)肝胆湿热证外周血单个核细胞的蛋白质表达谱的差异,为探讨清肝利湿汤的作用机制奠定基础.方法:利用双向凝胶电泳技术(2-DE)分离来源20例CHB肝胆湿热证患者用清肝利湿汤治疗前后的外周静脉血单个核细胞(peripheral blood mononuclearcells,PBMC).用基质辅助激光解吸飞行时间质谱(MALDI-TOF-MS)对用清肝利湿汤治疗前后的差异蛋白质进行测定肽质量指纹谱.检索MSDB和Swiss-Prot蛋白质数据库鉴定差异蛋白.结果:经过对比分析及质谱鉴定,找到质和/或量有明显改变的38个点.本研究鉴定了9个清肝利湿汤治疗前后的CHB患者的差异表达蛋白:Rho鸟苷酸解离抑制因子,热休克蛋白70,纤维蛋白gamma链前体,F肌动蛋白alpha链亚单位,纤维蛋白beta链前体,XTP3TPA反式激活蛋白,假想蛋白,丙酮酸激酶同工酶M1/M2,假想蛋白片段.结论:这些差异蛋白有助于了解CHB肝胆湿热证患者病理机制过程及清肝利湿汤的作用机制.AIM： To investigate the pharmacological mechanisms of qingganlishi decoction on patients with chronic hepatitis B （CHB） of liver dampness heat type using proteomic methods. METHODS： The total proteins of peripheral blood mononuclear cells from 20 cases before and after treatment of the qingganlishi decoction were separated by two-dimensional electrophoresis （2-DE） individually, and the differentially expressed protein spots between the two groups were identified by both matrix assisted laser desorption Pionization-time of flight-mass spec- trometry （MALDI-TOF-MS） so as to measure peptide mass fingerprinting. MSDB and Swiss- Prot protein database were retrieved to identify differentially expressed protein. RESULTS： Thirty eight spots were found to have marked changes on the quality and/or quantity. Among them, 9 proteins had been proved to be significantly up-regulated in the untreated CHB group but down-regulated after qingganlishi decoction treatment. They were listed as follows： Rho GDP-dissociation inhibitor 2, heat-shock 70 kDa protein, fibrinogen gamma-A chain precursor, F-actin capping protein alpha-1 subunit, fibrinogen beta chain precursor, XTP3TPA-transactivated protein 1, hypothetical protein, pyruvate kinase isozymes M1/M2, hypothetical protein fragment. CONCLUSION： These differentially expressed proteins of the CHB can help people under- stand the pathomechanism of hepatitis B of liver dampness heat type and the working mechanism of qingganlishi decoction.

关 键 词：蛋白质组学 清肝利湿汤 慢性乙型肝炎 肝胆湿热证 单个核细胞 双向凝胶电泳技术 

分 类 号：R259[医药卫生—中西医结合]