奶牛GV期卵母细胞OPS法和GMP法超低温冷冻效果  

Vitrification Effect of GV Stage Oocytes of Dairy Cows

作　　者：王珂[1] 郭海龙[1] 桑国俊[1] 赵兴绪[2] 

机构地区：[1]甘肃省畜牧兽医研究所,甘肃平凉744000 [2]甘肃农业大学动物医学院,甘肃兰州700070

出　　处：《动物医学进展》2009年第3期40-43,共4页Progress In Veterinary Medicine

基　　金：甘肃省生物工程项目;“牛胚胎规模化生产及相关新技术开发”项目

摘　　要：采用OPS管和GMP管对GV期的牛的卵母细胞进行玻璃化冷冻。在不同的前处理液中平衡5min,然后在冷冻液(EFS30,EFS40,EDFS30或EDFS40)中平衡30s,进行OPS法和GMP法玻璃化冷冻保存。结果显示,OPS法用EFS40液和EDFS40液冷冻后形态正常卵率为69.6%和76.1%,2组差异显著(P<0.05),成熟率最高达19.2%和33.3%,2组差异显著(P<0.05);GMP法用EFS40液和EDFS40液冷冻后形态正常卵率最高达75.6%和80.8%,2组差异显著(P<0.05),成熟率最高达15.6%和34.9%,2组差异显著(P<0.05)。而采用EDFS40液,OPS法和GMP法对GV期卵母细胞体外发育的影响差异均不显著,但GMP法的冷冻效率较高。表明采用EDFS40液GMP法对GV期卵母细胞的冷冻效率优于OPS法。Cow GV（germinal vesicle） stage oocytes were respectively vitrified in EFS30, EFS40, EDFS30 and EDFS40 by a two-step method with the different carrier（OPS＆GMP）. Kept in pre-balance solution in 5 minutes and different frozen solutions in 30 s, the cow GV oocytes were vitrified with OPS＆GMP. In cow GV oocytes vitrified by OPS, 69.6% oocytes were morphologically normal in EFS40 solution after thawing and 76.1% in EDFS40 solution （P〈0.05）. The mature rate was 17.2% in EFS40 solution and 35% in EDFS40 solution （P〈0.05）. While in bovine＇s GV oocytes vitrified by GMP, 75.6% oocytes were morphologically normal in EFS40 solution and 80.8% in EDFS40 solution （P〈0.05）, the mature rate was 15.6% in EFS40 solution after thawing and 34.9% in EDFS40 solution （P〈0.05）. Effects of carrier （OPS＆GMP） on verifications of cow GV oocytes were not significantly different （P〉0.05）,but effects of cow GV oocytes vitrified in EDFS40 by the two-step method with GMP were better than that in others.

关 键 词：奶牛 GV期卵母细胞 玻璃化冷冻 

分 类 号：S814.8[农业科学—畜牧学]