雄激素受体对前列腺癌PC3细胞株增殖能力的影响  

Influence of androgen recepter to prostate cancer cell line PC3 proliferation

作　　者：于胜强[1] 史本康[2] 张克勤[2] 

机构地区：[1]上海交通大学附属第一人民医院,上海200080 [2]山东大学齐鲁医院

出　　处：《山东医药》2009年第6期26-28,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(30672109)

摘　　要：目的研究雄激素受体(AR)对雄激素非依赖性前列腺癌细胞株PC3增殖能力的影响。方法将含有AR的质粒(Pbabe-AR)稳定转染到PC3细胞,建立起新的细胞系PC3-AR,并用实时逆转录聚合酶链反应(Q-PCR)、蛋白免疫印迹试验(Westernblot)和荧光素酶法确认AR的表达水平和功能;用MTT法、软琼脂糖凝胶实验检测AR对体外培养PC3细胞增殖的影响。结果PC3-AR细胞能够稳定表达有功能的AR;MTT实验显示PC3-AR细胞体外生长慢于PC3-v细胞,加入DHT以后效果更加明显。相对于PC3-v,PC3-AR在软琼脂糖凝胶中形成的克隆明显减少。结论AR能够降低PC3-AR细胞在体外的增殖能力。Objective To investigate the influence of androgen receptor （AR） to prostate cancer cell line PC3 proliferation. Methods The PC3-AR cells were established by transfecting the plasmid （Pbabe-AR） to the PC3 cells. The real-time RT-PCR, Western-blot, and reporter gene assay （Luciferase assay） were performed to detected the AR level and function in PC3-AR cells. The MTr and soft agar assay were performed to determine the in vitro growth ability of PC3 -v and PC3-AR cells. Results PC3-AR cells could express a functional AR. Compared with PC3-v cells, PC3-AR cells grew slower. After treating with DHT, PC3-AR cells grew even much slower. In soft agar assay, PC3-AR cells formed much fewer and smaller colonies than PC3-v cells. Conclusions AR can inhibit PC3 cells proliferation in vitro.

关 键 词：雄激素 雄激素受体 前列腺肿瘤 前列腺癌 细胞增殖 

分 类 号：R697.3[医药卫生—泌尿科学]