斑茅SAMDC基因的克隆及其原核表达分析  

Construction of Prokaryotic Expression Vector of Erianthus Arundinaceus Samdc and Its Expression in Escherichia Coil

作　　者：蔡秋华[1,2] 张积森[1] 刘文荣[1] 饶进[1] 翁笑艳[1] 陈如凯[1] 张木清[1] 

机构地区：[1]福建农林大学农业部甘蔗生理生态与遗传改良重点开放实验室,福建福州350002 [2]福建省农业科学院水稻研究所,农业部闽台农作物种质资源利用重点开放实验室,福建福州350019

出　　处：《福建稻麦科技》2009年第1期17-20,共4页Fujian Science and Technology of Rice and Wheat

摘　　要：以斑茅(Erianthus arundinaceus)samdc基因的cDNA为基础,采用基因重组技术,将该基因按正确的阅读框架定向克隆于原核表达载体pET-29a(+)中,转化大肠杆菌BL21(DE3),用IPTG诱导表达,并对表达产物进行SDS-PAGE分析。结果表明:重组斑茅samdc基因在大肠杆菌中获得高效表达,其分子量约为43.281kDa。斑茅samdc基因原核表达载体的成功构建和重组斑茅SAMDC蛋白在大肠杆菌中的高效表达,为进一步研究其生物学功能奠定了基础。Prokaryotic expression vector pET-SAMDC of Erianthus arundinaceus samdc gene cDNA was constructed by means of recombinant gene technology and expressed in Escherichia coil BL21 under induction of IPTG, the expressed products were detected by SDS-PAGE analysis. Result show： Recombinant samdc was efficiently expressed in E.coli BL21,it molecular weight was about 43.281Kda. The successful construction of prokaryotic expression vector containing Erianthus arundinaceus samdc gene and effective expression of recombinant SAMDC in E.coli BL21 laid the foundation for further study on its biological function.

关 键 词：斑茅 SAMDC 表达载体构建 基因表达 

分 类 号：S566.1[农业科学—作物学]