西太平洋暖池区深海沉积物细菌群落结构分析  

Phylogeny of Bacteria in Deep-sea Sediments from West Pacific Warm Pool

作　　者：王鹏[1] 肖湘[2] 王风平[2] 

机构地区：[1]同济大学海洋地质国家重点实验室,上海200092 [2]国家海洋局第三海洋研究所生物工程重点实验室,厦门361005

出　　处：《同济大学学报（自然科学版）》2009年第3期404-409,共6页Journal of Tongji University:Natural Science

基　　金：国家自然科学基金资助项目(40606032);国家"973"重点基础研究发展规划资助项目(2007CB815904)

摘　　要：将传统培养方法与分子生物学方法结合,选取西太平洋暖池区上的5个位点进行深海细菌群落结构分析.变形梯度凝胶电泳(DGGE)带型分析表明5个位点的细菌群落结构相似,分子生物学调查得到的15种类型归属于变形细菌(Proteobacteria)的gamma亚群、alpha亚群、beta亚群、delta亚群,其中与硫代谢相关的克隆子数可达14.03%,说明该海域的硫代谢活动较活跃;用培养的方法共获得32株细菌,归属于变形细菌的gamma亚群、beta亚群,革兰氏阳性细菌.两种方法中变形细菌的gamma亚群为沉积物中的优势细菌类群,说明gamma-proteobacteria在太平洋海域有广泛的分布.分离的菌株大多能够在低温环境下产生大分子物质水解酶类,同样说明所调查海域有比较活跃的物质代谢活动.Phylogenetic studies based on 16S rDNA gene sequence analysis, together with bacterial cultivation were used to study the bacteria diversity in five sites of deep sea sediment from west Pacific warm pool. Denaturing gradient gel electrophoresis（DGGE） profiles reveal the similar bacteria populations in sediments from five sites. 16S rDNA gene sequence analyses reveal that all clones fall in proteobacteria （in subdivision gamma-, alpha-, beta-, delta-）, 14.03% clones are related with sulfur metabolism, indicating a relative high activity of sulfur metabolism; 32 strains isolated from the samples belong to two divisions： proteobacteria （in subdivision gamma-, beta-） and gram-positive bacteria.Gamma-proteobacteria dominated the bacterial community of five sites indicates the widely distributed of gamma- proteobacteria in Pacific Ocean. Majority of the bacterial strains are able to secrete diversity cold adaptive hydrolytic enzymes into the medium at 4 ℃, indicates the relatively high metabolic activity in west Pacific Ocean.

关 键 词：深海沉积物 西太平洋 暖池区 微生物 多样性 

分 类 号：Q93[生物学—微生物学]