栀子HPLC数字化指纹图谱相对定量方法研究  

The relative quantitative method of HPLC digitized fingerprints for Fructus Gardeniae

作　　者：侯志飞[1] 孙国祥[2] 

机构地区：[1]河北化工医药职业技术学院制药工程系,河北石家庄050026 [2]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《中成药》2009年第3期329-335,共7页Chinese Traditional Patent Medicine

摘　　要：目的:建立栀子HPLC数字化指纹图谱并以对照药材为参照对相对定量方法进行研究。方法:采用反相高效液相色谱法,使用CenturySIL C18BDS柱(20 cm×4.6 mm,5μm),以1%醋酸水-1%醋酸乙腈为流动相,低压线性梯度洗脱,检测波长265 nm,柱温(30.0±0.15)℃,测定10批不同产地栀子HPLC指纹图谱和栀子对照药材指纹图谱。以栀子对照药材供试液不同进样量时各指纹峰的峰面积分别对表观进样量(mg)进行线性回归,用"中药色谱指纹图谱超信息特征数字化评价系统"软件计算得对照药材折合标准曲线。结果:以栀子苷峰为参照物峰,确定35个共有峰,建立了栀子HPLC数字化指纹图谱。以栀子对照药材为参照计算出各产地栀子相当于对照药材的折合百分含量以及所有指纹成分相当于对照药材中相应成分的折合百分含量。结论:所建立的指纹图谱相对定量方法可衡量不同产地栀子药材中指纹成分的含量,可作为栀子药材质量控制的依据。该方法对深入研究单一指纹峰的相对含量具有较重要意义,也为丰富中药色谱指纹图谱的定量评价方法提供了重要参考价值。AIM： To explore a relative quantitative method by establishing HPLC digitized fingerprints of Fructus Gardeniae and taking the control medicinal material as a reference substance. METHODS： The chromatographic fingerprints were obtained by injecting the sample solution each time on a CenturySIL C18 BDS column （20 cm × 4.6 mm, 5 μm） with the gradient elution solvent system composed of 1% acetate acid-water and 1% acetate acidacetonitrile. The flow rate was 1.0 mL/min, the column temperature was maintained at （30 ± 0.15 ） ℃ and the detection wavelength was set at 265 nm. The HPLC fingerprints of the control medicinal material for Fructus Gardeniae were obtained under different injection volumes and calculated by ＂the digitized evaluation system of the traditional Chinese medicine fingerprint with the super-information characteristics＂. Equivalent regression curves were obained by making linear regression between the fingerprint peak areas and apparent injection amounts. RESULTS： 35 co-possessing peaks were selected as the fingerprint peaks of Fructus Gardeniae by taking Geniposide peak as the reference peak. Both the equivalent relation between each of the ten places and control medicinal material of Fructus Gardeniae and the equivalent relation between each fingerprint peak of different places and that of Fructus Gardeniae control medicinal material were calculated by equivalent regression curves. CONCLUSION： This method can be useful in the quality control of Fructus Gardeniae. It is also of importantance in deeply studying the relative amounts of single fingerprint peak, and it provides reference value in extending the quantitative evaluation methods of TCM.

关 键 词：栀子 HPLC数字化指纹图谱 对照药材折合标准曲线 折合百分含量 

分 类 号：R284.1[医药卫生—中药学]