肝络通对大鼠肝纤维化门脉高压症的药效学研究  

Effects of Glytan in rats on portal hypertension

作　　者：韩琳[1] 杜庆红[1] 姜俊杰[1] 王筠[1] 侯金才[1] 雷洪涛[1] 李澎涛[1] 

机构地区：[1]北京中医药大学基础医学院人体形态系,北京100029

出　　处：《中成药》2009年第3期350-354,共5页Chinese Traditional Patent Medicine

基　　金："教育部长江学者和创新团队发展计划"项目(RIRT0413);中国博士后科学基金资助项目(20080430341)

摘　　要：目的:研究肝络通(甘草酸二铵、丹酚酸B)对胆管阻塞性肝纤维化模型大鼠门脉高压症的治疗作用。方法:采用胆总管结扎方法复制肝纤维化门脉高压症模型,造模成功一周后随机分为5组:模型、高、中、低剂量组和心得安组,并设假手术组,分别灌胃给药。于术后2周和4周分别测定各组的门静脉压力、肝功能、羟脯氨酸含量并进行病理组织学检查。结果:肝络通及心得安各治疗组大鼠门静脉压力较模型组明显降低,但肝络通各组与心得安组比较无显著性差异。肝络通能显著降低血清AST、ALT、TBIL和Na+的浓度,同时降低肝组织羟脯氨酸蛋白含量。术后4周HE染色显示肝络通能显著抑制胶原生成,促进肝组织结构的恢复。结论:肝络通能减缓肝纤维化进程对肝脏起到保护作用。AIM ： To observe the effects of Glytan （ diammonium alycyrrhizinate, salvianolic acid B） on rat portal hypertension caused by hepatic fibrosis. METHODS： Portal hypertension models were made by bile duct ligation （BDL）. One week after operation, BDL model rats were randomly divided into five groups： a model group, three Glytan groups, a Propranolo Hydrochloride group, and sham-operation group. All the rats were treated respectively. At the end of 2 and 4 weeks, the portal pressure, hepatic function, the contents of hydroxyproline and the histopathologic change of hepatic tissue were detected in six groups. RESULTS ： The portal pressure of the rats in Glytan groups and Propranolo Hydrochloride group were higher than that in the model group, but there was no significant difference between Glytan groups and Propranolo Hydrochloride group. Glytan degraded the concentration of AST, ALT, TBIL, and Na^ ＋ concentration in blood-serum significantly and at the same time downregulated the contents of hydroxyproline in livers. After 4 weeks, HE staining showed that Glytan inhibited collagenatous synthesis, promoted the recovery of hepatic tissue. CONCLUSION ： Glytan can inhibite the progress of liver fibrosis.

关 键 词：肝络通 门脉高压症 药效 大鼠 

分 类 号：R285.6[医药卫生—中药学]