抗癌生物活性肽对实验性胃癌PCNA、caspase-8基因表达的影响  

INFLUENCE OF THE PCNA AND CASPASE-8 EXPRESSION OF ANTI-CANCER BIOACTIVE PEPTIDE ON EXPERIMENTAL GASTRIC CANCER

作　　者：王玉芳[1] 闫美荣[2] 贾淑芹[2] 刘桂荣[2] 苏秀兰[2] 

机构地区：[1]内蒙古医学院附属医院病理科,内蒙古呼和浩特010050 [2]内蒙古医学院临床医学研究中心

出　　处：《内蒙古医学院学报》2009年第1期6-9,共4页Acta Academiae Medicinae Neimongol

基　　金：国家自然科学基金(30460146)

摘　　要：目的:探讨抗癌生物活性肽作用于荷胃癌裸鼠后,对其增殖及凋亡相关基因表达变化的影响。方法:将裸鼠接种胃癌MGC-803细胞后使之致瘤。实验随机分为3组,对照组11只,脾肽实验组12只,肝肽实验组11只;分别尾静脉注射生理盐水0.4mL、脾肽0.4mL(0.15mg/mL)、肝肽0.4mL(0.05mg/mL)。连续处理35d,停药4d后处死裸鼠,取肿瘤,以4%多聚甲醛固定,常规石蜡包埋,4μm切片。应用免疫组化SABC法检测PCNA、caspase-8蛋白在实验组与对照组中的表达情况。结果:PCNA蛋白在对照组(NS组)肿瘤中阳性表达较强,在P组、G组裸鼠肿瘤中PCNA蛋白的表达均比对照组的表达弱,且有显著性差异(P<0.01);caspas-8蛋白在对照组(NS组)肿瘤中阳性表达较弱,P、G组裸鼠肿瘤中caspas-8蛋白的表达均比对照组表达强,且有显著性差异(P<0.01)。结论:抗癌生物活性肽能降低胃癌移植瘤细胞的增殖活性,并能促进其凋亡。Objective：To investigate the expression changes of proliferating and apoptotic related genes of anti-cancer bioactive peptide treated tumor-loaded nude mice.Methods：Gastric cancer cell line MGC-803 was inoculated to nude mice to make them tumor-loaded.Nude mice were divided into 3 groups randomly：11 as control group,12 as spleen peptide group and 11 as liver peptide group.3 groups of nude mice were administered with 0.4mL normal saline,0.4mL spleen peptide（0.15mg/mL）and 0.4mL liver peptide（0.05mg/mL）continuously for 35 days,respectively.Nude mice were executed after the administration finished 4 days later,tumors were taken out and fixed with 4% paraformaldehyde,routine paraffin embedded and sliced into 4μm.Then,PCNA and Caspase-8 expression were detected using immunohistochemistry SABC method between experimental and control groups.Results：The expression of PCNA was relatively stronger in control group than those in experimental groups which showed significant difference（P〈0.01）.While the expression of Caspase-8 was relatively weaker in control group than that in experimental groups and which showed significant difference（P〈0.01）.Conclusion：Anti-cancer bioactive peptide can inhibit the proliferating activity of transplanted gastric cancer cells and promote cell apoptosis.

关 键 词：胃癌 裸鼠 增殖 凋亡 抗癌生物活性肽 

分 类 号：R392.11[医药卫生—免疫学]