体外诱导骨髓间充质干细胞向神经元细胞分化的实验研究  

DIFFERENTIATION OF MESENCHYMAL STEM CELL INTO NEURON-LIKE CELL IN VITRO

作　　者：马庆海[1] 武文元[1] 张占普[1] 王飞[1] 

机构地区：[1]内蒙古医学院附属医院神经外科,内蒙古呼和浩特010050

出　　处：《内蒙古医学院学报》2009年第1期19-23,共5页Acta Academiae Medicinae Neimongol

基　　金：内蒙古自治区教育厅资助项目(NJ02084)

摘　　要：目的:体外分离培养骨髓间充质干细胞(MSC),并诱导其向神经元细胞分化。方法:分离大鼠骨髓间充质干细胞,并于体外扩增、培养,并通过诱导培养基进行体外诱导培养,比较对照组及实验组间细胞形态和神经丝蛋白(NFP)表达率的不同。结果:诱导后,实验组中80%的细胞表现出神经元样形态,且NFP阳性表达率明显高于对照组。结论:MSC在体外可以被诱导为神经元样细胞。Objective：Mesenchymal stem cell（MSC）was isolated and grown in vitro,and induced to be differentiated into neuron-like cell.Methods：MSC was isolated,grown and induced in vitro.The comparison of induced cell morphology and expression of neurofilament protein（NFP） was carried out between control and experimental groups.Results：More than 80% of MSC exhibited neuron-like characteristics.NFP-positive cell in experimental group is higher than that in the control group（P〈0.05）.Conclusion：MSC can be induced to differentiate into neuron-like cell in vitro.

关 键 词：骨髓间充质干细胞 诱导 分化 神经元样细胞 

分 类 号：Q813.11[生物学—生物工程]