卷丹百合种间杂种胚培养研究  

Embryo Culture of Interspecific Hybrids from Lilium lancifolium Thunb.

作　　者：雷家军[1] 庞兰[1] 林翼飞[1] 关婧竹[1] 

机构地区：[1]沈阳农业大学园艺学院,沈阳110161

出　　处：《沈阳农业大学学报》2009年第1期84-87,共4页Journal of Shenyang Agricultural University

基　　金：辽宁省教育厅基金项目(202053092)

摘　　要：以三倍体卷丹百合(Lilium lancifolium Thunb.)为母本与兰州百合等7种百合进行种间杂交,以所得幼胚为外植体进行离体培养,研究不同杂交组合、消毒方法、幼胚处理方法、胚龄、激素配比对杂种胚培养的影响。结果表明:卷丹×大花卷丹有胚率最高,达41.3%;未开裂蒴果用70%酒精浸泡1min消毒效果最好;剥除种皮和胚乳接种幼胚的萌发率比不剥除高60%～70%,且萌发时间提早17～22d;授粉后40～50d的杂种胚最适于胚培养;最适幼胚萌发培养基为MS+NAA0.01mg·L-1+6-BA0.1～0.5mg·L-1。The immature embryos obtained from interspecific hybridization from Lilium lancifolium as a female parent with 7 other lily species were used as the explants to tissue culture. The influencing factors of embryo culture were studied in this paper. The results indicated that the percentage of seeds with embryo of L. lancifolium × L. maximowiczii was the highest, with up to 41.3%. 70% alcohol for 1 minute was the best disinfection method for capsules without dehiscence. The rate of germination of embryos without seed coat and endosperm was 60%-70% higher than that with seed coat and endosperm, and germinated 17-22 days earlier. The best age for the immature embryos to culture in vitro was in 40-50 days after pollination. The best medium for embryos germination was MS＋NAA0.01 mg.L^-1 ＋6-BA0.1 mg.L^-1.

关 键 词：卷丹 百合 种间杂交 幼胚 离体培养 

分 类 号：S682.2[农业科学—观赏园艺]