HIV-2 env 基因在重组痘苗病毒中的表达  

Expression of HIV 2 Envelope Gene in Recombinant Vaccinia Virus

作　　者：田梅[1,2] 金宁一[1,2] 王秀清[1,2] 郭志儒[1,2] 方厚华[1,2] 范洪学[1,2] 殷震[1,2] 

机构地区：[1]解放军农牧大学军事兽医研究所 [2]白求恩医科大学

出　　处：《高技术通讯》1998年第4期48-52,共5页Chinese High Technology Letters

基　　金：总后卫生部重点资助项目

摘　　要：以痘苗病毒复制非必需区血凝素（ＨＡ）基因为侧翼，将人Ⅱ型免疫缺陷病毒ｇｐ１２０和ｇｐ３６基因分别置于不同类型的自然型痘苗病毒复合启动子下游，构建了４种表达质粒，经同源重组和血凝素阴性空斑筛选，获得了４株重组痘苗病毒，经免疫荧光试验和Ｗｅｓｔｅｒｎｂｌｏｔ检测证明，４株重组病毒均能表达目的蛋白。在重组病毒感染早期、晚期，不同株表达量略有不同，表达产物分子量分别约为５７ｋＤａ和４２ｋＤａ，具有良好的反应原性。With flank sequence of HA gene of vaccinia virus, four recombinant virus expression plasmids containing cDNA of human immunodeficiency virus type II surface glycoprotein and transmembrane protein gene under the control of different hybird promoters of pox virus were constructed, and transfected into BHK 21 cells which were infected by wild type vaccinia virus. The four recombinant vaccinia viruses vRJ1 16gp36, vRJ2 16gp36, vRJ1 16gp120 and vRJ2 16gp120 were screened and cloned by hemadsorption in BHK 21 cells. Indirect immunofluorescence assay(IFA) and Western blot showed that the molecular weight were about 57kDa and 42kDa. In the early stage of infection, the production of four rVVs doesn′t show any notable discrepancy, while in late stage, vRJ2 16gp36 and vRJ2 16gp120 were higher than vRJ1 16gp36 and vRJ1 16gp120 respectedly. The four rVVs all can be recognized by sera of HIV 2 infected patients.

关 键 词：HIV-2 ENV基因 重组痘苗病毒 表达 

分 类 号：R373.12[医药卫生—病原生物学]