镧对酸雨胁迫下高粱种子萌发及POD活性和MDA含量的影响  

Effect of lanthanum on the germination and peroxidase activity in sorghum under acid-rain stress

作　　者：邱琳[1,2] 王娜[1] 周青[3,1] 

机构地区：[1]江南大学环境与土木工程学院,无锡214122 [2]浙江省环境科学保护设计研究院生态工程中心,杭州310007 [3]江南大学工业生物技术教育部重点实验室,无锡214122

出　　处：《中国生态农业学报》2009年第2期343-347,共5页Chinese Journal of Eco-Agriculture

基　　金：江苏省教育厅"江苏省高等学校大学生实践创新训练"项目(20070730)资助

摘　　要：本文以高粱"熊杂9号"为对象,采用模拟酸雨伤害的常规种子实验方法,研究稀土镧对酸雨胁迫下高粱种子萌发及过氧化物酶(POD)活性和丙二醛(MDA)含量的影响。结果显示,12mg·L01La(Ⅲ)浸种后,可明显缓解酸雨对高粱种子萌发的抑制作用,高粱种子发芽率、发芽势、发芽指数和活力指数较未处理组升高,而异状发芽率降低,pH2.5时变化幅度最明显。pH2.0时由于酸雨使种子内部结构严重破坏,La(Ⅲ)的加入已无法缓解酸雨引发的伤害。La(Ⅲ)处理组MDA含量和POD活性均低于未处理组。上述结果表明,La(Ⅲ)可通过调节POD活性减轻酸雨胁迫引发的膜脂过氧化,缓解酸雨对种子萌发的抑制作用。The effect of lanthanum on the germination, MDA content and peroxidase （POD） activity in sorghum seeds under acid-rain stress were investigated. The objective of the study was to develop a scientific method and technical guide for mitigating acid-rain damage on seed germination. Results show that sorghum seeds germinate best when seed soaked in 12 mg·L^-1 La（Ⅲ） solution, damage on seed germination from acid-rain decreases. Germination rate, germination energy, germination index, vigor in- dex are all higher than untreated seeds. Abnormal germination in treated seeds also tends to be lower than untreated, especially at pH 2.5. Because internal structure of seeds is destroyed by acid-rain with pH 2.0, La（Ⅲ） cannot reverse damage by acid-rain at pH 2.0. MDA content and POD activity of La（Ⅲ） treatment groups are lower than the non-La（Ⅲ） treatments, especially at pH 2.5 and 2.0. This indicates that La（Ⅲ） probably decreases membrane lipid peroxidation induced by acid-rain through POD activityadjustment, which eventually alleviates acid-rain damage on seed germination.

关 键 词：镧 酸雨胁迫 高粱种子 过氧化物酶 丙二醛 萌发 

分 类 号：X517[环境科学与工程—环境工程] Q945.34[生物学—植物学]