陆地棉栽培品种新陆早1号转基因抗虫植株的获得  

Obtaining of Insecticidal Transgenic Cotton Plants Derived from Upland Cotton Cultivar Xinluzao 1

作　　者：王伟[1] 朱祯[1] 邓朝阳[1] 高越峰[1] 徐鸿林[1] 肖桂芳[1] 郭仲琛 李向辉[1] 

机构地区：[1]中国科学院遗传研究所

出　　处：《高技术通讯》1998年第5期1-5,共5页Chinese High Technology Letters

基　　金：863计划;"总理基金";国家自然科学基金

摘　　要：通过根农杆菌介导法成功地将修饰的豇豆胰蛋白酶抑制剂（ＣｐＴＩ）基因导入新疆陆地棉栽培品种新陆早１号。棉花幼苗下胚轴经根农杆菌共培养、抗性愈伤组织筛选和胚性愈伤的诱导等阶段获得了胚状体；经ＥＬＩＳＡ检测选择Ｎｐｔ－Ⅱ阳性的克隆进行分化培养和植株再生；经ＥＬＩＳＡ再度检测，选择Ｎｐｔ－Ⅱ阳性的再生植株进一步培养。当生长至５—６片真叶时，将再生植株直接移栽到温室或进行嫁接。通过ＰＣＲ鉴定和ＰＣＲＳｏｕｔｈｅｒｎ杂交检测证明，修饰的ＣｐＴＩ基因已存在于再生植株的基因组内。抗虫测试结果表明，转化棉株对棉铃虫具有较强的抗性。The modified cowpea tyrypsin inhibitor(SCK) gene was successfully transferred into Xinjiang upland cotton cultivar Xinluzao 1 (Gossypium hirsutum L.) by Agrobacterium mediated transformation. After co cultivation of hypocotyl segment of seedling with Agrobacterium harbored SCK gene, the kanamycin resistant calli were secreened and the embryos were induced following culturing on selective medium and embryo inducing medium. The transformed colonies were selected by Npt Ⅱ ELISA and used in plant regeneration. The plantlets with 5_6 real leaves were obtained 2_3 months after culturing on differentiation medium and transplanted to pot or grafted. PCR and PCR Southern hybridization confirmed the presence of foreign SCK gene in transgenic plants. The results of bioassay demonstrated that transgenic cotton shows notable inhibitory effects to the larvae of cotton bollworm (Heliothis armigera Hubner).

关 键 词：抗虫基因 基因修饰 陆地棉 根农杆菌 棉花 

分 类 号：S562.034[农业科学—作物学]