凋亡相关新基因 TFAR19 的 cDNA 克隆、表达和功能研究  

Study of cDNA Cloning, Expression and Function of a Novel Apoptosis related Gene TFAR19

作　　者：刘红涛[1] 王玉刚[1] 张颍妹 宋泉声[1] 狄春晖 陈光慧[1] 汤建[1] 马大龙[1] 

机构地区：[1]北京医科大学卫生部医学免疫学重点实验室

出　　处：《高技术通讯》1998年第5期6-10,共5页Chinese High Technology Letters

基　　金：863计划资助项目

摘　　要：利用ＲＤＡ技术从人白血病细胞ＴＦ－１中克隆成功一个与凋亡相关的新基因ＴＦＡＲ１９。序列分析表明，ＴＦＡＲ１９ｃＤＮＡ全长５５９ｂｐ，其中２５—３９９ｂｐ编码１２５个氨基酸的蛋白质。ｍＲＮＡ斑点杂交分析表明ＴＦＡＲ１９在５０种组织中均有不同程度的表达。在大肠杆菌中表达并纯化了重组ＴＦＡＲ１９蛋白质，制备了多克隆抗体，ＷｅｓｔｅｒｎＢｌｏｔ发现ＴＦＡＲ１９蛋白在凋亡的ＴＦ－１细胞中高表达。瞬时转染ＴＦＡＲ１９正义基因后，ＴＦ－１细胞去细胞因子后凋亡速度增加。研究表明它能抑制胃癌细胞株８０３细胞和Ｈｅｌａ细胞的生长，促进它们的去血清所致的凋亡。A novel apoptosis ralated gene is cloned and named TFAR19(TF 1 apoptosis related clone19). The full length of TFAR19 cDNA is 559bp and the 25—399bp encodes 125 amino acid ORF. The TFAR19 mRNA expresses in hardly all adult human tissues, and its expression in fetal tissues is significantly lower than that in adult. TFAR19 is expressed and purified in E. coli and the TFAR19 protein expressed highly in the apoptosis of TF 1 detected by Western Blot. After transient transinfection of TFAR19, the apoptosis of TF 1 cell is enhanced. The results show that overexpression of TFAR19 could inhibit the growth of two tumor cells (803 cell from human stomach tumor and Hela cell) and enhance the apoptosis of 803 and Hela cell induced by deprivation of serum in the culture medium. All the results show that TFAR19 might be an enhancer of apoptosis of some tumor cells under the condition of deprivation of growth factor.

关 键 词：TF-1细胞 凋亡 CDNA 免疫学 

分 类 号：R392.12[医药卫生—免疫学]