纳米颗粒-葡萄糖与肿瘤细胞的相互作用  

The Interaction between Cancer Cells and Nanoparticles-glucose Bioconjugates(FSiNPs-DG)

作　　者：霍希琴[1,2,3] 霍海玲[1,2] 伍旭[2,4] 何晓晓[1,2,3] 

机构地区：[1]湖南大学化学化工学院,湖南长沙410082 [2]湖南大学化学生物传感与计量学国家重点实验室,湖南长沙410082 [3]湖南大学生物医学工程中心,湖南长沙410082 [4]湖南大学生命科学与技术研究院,湖南长沙410082

出　　处：《湖南大学学报（自然科学版）》2009年第3期67-70,共4页Journal of Hunan University:Natural Sciences

基　　金：教育部新世纪优秀人才支持计划资助项目(NCET-06-0697);国家自然科学基金资助项目(90606003;20775021);湖南省杰出青年基金资助项目(06JJ10004);湖南省科技厅科技计划资助项目(2008KJ3205)

摘　　要：通过物理吸附法制备了硅壳荧光纳米颗粒-氨基葡萄糖复合物(FSiNPs-DG),并选择三株肿瘤细胞和一株正常细胞作为考察分析对象,基于肿瘤细胞表面高表达的对葡萄糖具有高亲和力的葡萄糖转运蛋白考察了FSiNPs-DG对固定后肿瘤细胞的识别情况,在此基础上进一步考察了FSiNPs-DG与活细胞的作用,即肿瘤细胞对FSiNPs-DG吞噬情况.结果表明:利用物理吸附法制备出的FSiNPs-DG可以较好地识别固定后的肿瘤细胞,并且肿瘤细胞对FSiNPs-DG吞噬量明显高于正常细胞.The silica coated fluorescent nanoparticles-glucosamine complexes （FSiNPs-DG） was prepared by physical adsorption. Three kinds of cancer cells and one kind of normal cells were selected for the analysis. The recognition of FSiNPs-DG to immobilize cancer cells was investigated based on the principle that glucose transportor is highly expressed on the membrance of cancer ceils. And then the uptake of FSiNPs-DG by the cancer cells was further investigated. The results have indicated that the FSiNPs-DG can recognize the immobilized cancer cells very well, and the uptake of FSiNPs-DG by cancer cells was more noticeable than the normal cells.

关 键 词：肿瘤细胞 硅壳荧光纳米颗粒 葡萄糖 

分 类 号：O636.1[理学—高分子化学]