肝细胞癌患者外周血中AFP-mRNA水平的检测  

Detection of AFP-mRNA levels in Peripheral Blood of Patients with Hepatocellular Carcinoma

作　　者：李林泽[1] 牛聪敏[2] 李友良[3] 

机构地区：[1]河北省保定市急救中心,071000 [2]保定市传染病医院 [3]保定市卫生局情报站

出　　处：《职业与健康》2009年第8期812-813,共2页Occupation and Health

摘　　要：目的探讨甲胎蛋白(AFP)-mRNA在原发性肝癌(HCC)患者外周血中的表达和临床意义。方法采用荧光定量逆转录聚合酶链反应(RT-PCR)方法,检测25例HCC患者外周血中AFP-mRNA的表达。结果HCC患者外周血中AFP-mRNA含量显著高于肝炎和肝硬化病人(P<0.01)。HCC患者外周血中AFP-mRNA含量与病人的肝外转移明显相关(P<0.05)。HCC患者外周血中AFP-mRNA含量与病人的临床分期肿瘤直径、数目、血清AFP水平无明显相关(P>0.05)。结论AFP-mRNA可作为血循环中有肝癌细胞或肝癌细胞的标志物,对HCC患者,阳性预示有血源性转移的可能,可作为预测肝癌复发、转移的参考指标。[ Objective] To investigate the expression of alpha-fetoproteins （AFP） in peripheral blood of patients with hepatocellular carcinoma （HCC）. [ Methods] RT-PCR was adopted to detect the expression of AFP-mRNA in peripheral blood of 25 patients with hepatocellular carcinoma （HCC）. [ Results] AFP-mRNA levels in patients with HCC were significant higher than that in patients with hepatitis and cirrhosis. AFP-mRNA levels did not significantly correlated with the clinical stage, tumor diameter, tumor amount, and serum AFP levels （ P 〉 0. 05）. [ Conclusion] AFP-mRNA can be the specific marker of hepatoma cells in blood circulation. For HCC patients, the positive AFP-mRNA indicates the possibility of hematogenous metastasis, which can serve as a reference indicator of prediction of liver cancer recurrence, and metastasis.

关 键 词：肝细胞肝癌 甲胎蛋白 MRNA 

分 类 号：R446.1[医药卫生—诊断学]