酸性大红GR极谱法测定牛血清白蛋白  

Determination of Bovine Serum Albumin by Acid Scarlet GR Polarography

作　　者：孟莉新[1] 杨海英[1] 

机构地区：[1]运城学院生化实验中心,山西运城044000

出　　处：《畜牧与饲料科学》2009年第2期13-14,共2页Animal Husbandry and Feed Science

摘　　要：笔者报道了在酸性大红GR(ASGR)存在下,高灵敏度测定牛血清白蛋白(BSA)的极谱方法。在pH值为7.43的Tris-HCl缓冲溶液中,ASGR在-0.88V(vs.SCE)处有一个灵敏的还原峰。加入BSA后,ASGR还原峰电位正移,且峰电流发生改变。当BSA的浓度高于ASGR浓度0.35倍时,ASGR峰电流下降;当BSA的浓度低于ASGR浓度0.35倍时,ASGR峰电流却增大。在选定的试验条件下,ASGR二阶导数还原峰峰电流值与所加BSA的浓度在1.0×10-9～9×10-7mol/L范围内呈良好的线性关系,可望用于痕量BSA的定量分析。While there was acid scarlet GR （AS GR）,the polarographic method of determining bovine serum albumin （BSA） with high sensitivity was reported in this article. In Tris-HCl buffer solution with pH value being 7.43,AS GR had a sensitive reduction peak at-0.88 V （vs.SCE）. After BSA was added,the reduction peak potential of AS GR was shifted positively and the peak current was changed. When the concn. of BSA was 0.35 times higher than that of AS GR,the peak current of AS GR was decreased; when the concn. of BSA was 0.35 times lower than that of AS GR,the peak current of AS GR was increased. Under the chosen experimental condition,the second order derivative peak current of reduction peak of AS GR showed good linear correlation with the concn. of added BSA in the range of 1.0 × 10-9 ～ 9.0×10-7 mol/L. This method could be applied in the quantitative analysis of trace BSA.

关 键 词：牛血清白蛋白 ASGR 极谱法 

分 类 号：O657.14[理学—分析化学]