鹅IGF-Ⅰ基因5′调控区单核苷酸多态性分析  

Polymorphism Analysis on the Single Nucleotide in the 5' Regulatory Region of IGF-1 Gene in Goose

作　　者：杜晓东[1] 张绍胜[1] 姜润深[1] 夏生林 耿照玉[1] 袁绍友[3] 

机构地区：[1]安徽农业大学动物科技学院,安徽合肥230036 [2]安徽省芜湖市动物疾控中心,安徽芜湖241000 [3]安徽省皖西白鹅原种场,安徽六安237005

出　　处：《畜牧与饲料科学》2009年第2期134-136,共3页Animal Husbandry and Feed Science

摘　　要：以皖西白鹅和朗德鹅为素材,设计引物对鹅的IGF-Ⅰ基因5′端非编码区序列克隆测序,运用PCR-SSCP方法检测IGF-Ⅰ基因5′调控区的单核苷酸多态性。检测发现该区域存在4个SNP位点,分别是:A-26T、A-215G、A-314G、A-325T。With Wanxi white geese and Landes geese as materials,the primers were designed for cloning and sequencing the sequence in 5＇ non-coding region of IGF-1 gene in goose. The polymorphism of single nucleotide in 5＇ regulatory region of IGF-1 gene was detected by PCR-SSCP method. It was found through detection that there were 4 SNP sites in the region and they were A-26T,A-215G,A-314G and A-325T resp.

关 键 词：鹅 IGF-Ⅰ基因 PCR-SSCP 单核苷酸多态性 调控区 

分 类 号：Q78[生物学—分子生物学]