超高效液相色谱-电喷雾电离串联质谱联用法检测茶叶中阿维菌素类药物残留  

Determination of avermectin residues in tea by ultra-performance liquid chromatography-electrospray tandem mass spectrometry

作　　者：杨方[1] 杨守深[2] 林永辉[1] 郑丹萍[2] 卢声宇[1] 黄志强[3] 张莹[3] 

机构地区：[1]福建出入境检验检疫局,福建福州350001 [2]福建农林大学,福建福州350003 [3]湖南出入境检验检疫局,湖南长沙410007

出　　处：《色谱》2009年第2期153-157,共5页Chinese Journal of Chromatography

基　　金：国家“十一五”科技支撑计划项目(No.2006BAK02A08)

摘　　要：建立了采用超高效液相色谱-电喷雾电离串联质谱同时检测茶叶中爱比菌素、甲胺基阿维菌素、乙酰胺基阿维菌素、伊维菌素、多拉菌素、莫西丁克残留的方法。试样经饱和氯化钠溶液浸润后,用乙腈提取,C18固相萃取小柱净化,超高效液相色谱-电喷雾串联质谱法（UPLC/ESI-MS/MS）测定。对流动相、监测离子、校正曲线等进行了优化和探讨。6种分析物在2.0～50μg/L范围内线性关系良好,相关系数均大于0.992 0。莫西丁克在5,10,20μg/kg,其余分析物在2,5,10μg/kg加标水平的平均回收率为61.7%～85.4%,相对标准偏差为9.37%～17.19%。该方法可靠、稳定,可满足茶叶中阿维菌素类药物残留检测与确证的需要。A simple and rapid analytical method for the simultaneous determination of abamectin,emamectin,eprinomectin,ivermectin,doramectin and moxidectin residues in tea by ultra-performance liquid chromatography-electrospray tandem mass spectrometry（UPLC/ESI-MS/MS） has been developed.The avermectins were extracted from the tea with acetonitrile after the tea was infiltrated in saturated aqueous NaCl solution,then cleaned up with a C18 solid phase extraction cartridge.The linear ranges were 2.0-50 μg/L and the correlation coefficients were all above 0.9920.Several UPLC-MS/MS conditions that included the mobile phase,monitor ions and the selection of calibration of the measurement were studied.The average recoveries and the relative standard deviations ranged from 61.7% to 85.4% and from 9.37% to 17.19%,respectively,in spiked samples at the concentrations of 5,10,20 μg/kg for moxidectin and 2,5,10 μg/kg for other analytes.This method is suitable for the determination of avermectin residues in tea.

关 键 词：超高效液相色谱-质谱联用法 阿维菌素类药物残留 茶叶 

分 类 号：O658[理学—分析化学]