鼠脑组织中6-羟基-1-甲基-1,2,3,4-四氢-β-咔啉及其相关生物胺的高效液相色谱-电化学检测  

Determination of 6-hydroxy-1-methyl-1,2,3,4-tetrahydro-β-carboline,5-hydroxytryptamine and 5-hydroxyindole acetic acid in the neonatal rat brains using high performance liquid chromatography-electrochemical detection

作　　者：毛健[1] 苏旸[1] 栾玉静[1] 陈薛钗[1] 邓玉林[1] 

机构地区：[1]北京理工大学生命科学与技术学院,北京100081

出　　处：《色谱》2009年第2期216-219,共4页Chinese Journal of Chromatography

基　　金：北京市科技计划项目(20070639034)

摘　　要：建立了大鼠脑组织中6-羟基-1-甲基-1,2,3,4-四氢-β-咔啉（6-OH-MTHβC）、5-羟色胺（5-HT）和5-羟吲哚乙酸（5-HIAA）含量的高效液相色谱-库仑阵列电化学检测（HPLC-ECD）方法。采用的色谱柱为Discovery（HS F5柱（250mm×4.6mm,5μm）,流动相为缓冲液（40mmol/L柠檬酸＋20mmol/L磷酸氢二钠＋0.3mmol/L乙二胺四乙酸二钠,pH4.0）-甲醇（体积比为78∶22）混合液,流速为1mL/min。6-OH-MTHβC、5-HT、5-HIAA在1.0~500.0μg/L范围内线性关系良好（r〉0.999 2）,检出限分别为0.56,0.26,0.53μg/L,日内和日间精密度（以相对标准偏差表示）均低于6.1%,回收率分别为87.1%~98.2%,87.0%~95.3%,90.1%~97.7%。用该方法检测新生7d的SD胎鼠脑内6-OH-MTHβC及5-HT、5-HIAA的含量,发现SD胎鼠在急性酒精中毒8h后6-OH-MTHβC显著上升（P〈0.05）;而5-HT和5-HIAA的含量有所下降,但无显著性差异。该法简便、稳定、灵敏度高,适用于测定鼠脑组织中6-OH-MTHβC和5-HT,5-HIAA含量的相关研究。A simple method was developed for the analysis of 6-hydroxy-1-methyl-1,2,3,4-tetrahydro-β-carboline（6-OH-MTHβC）,5-hydroxytryptamine（5-HT） and 5-hydroxyindole acetic acid（5-HIAA） in rat brain by high performance liquid chromatography with electrochemical detection（HPLC-ECD）.The separation of the sample was performed on a Discovery HS F5 column（250 mm×4.6 mm,5 μm） with a mobile phase of the buffer（40 mmol/L citric acid＋20 mmol/L Na2HPO4＋0.3 mmol/L EDTA2Na,pH 4.0）-methanol（78∶22,v/v） at a flow rate of 1.0 mL/min.The electrochemical detector was Coularray Detector-4.This method showed good linearity（r〉0.9992） for the quantification of 6-OH-MTHβC,5-HT and 5-HIAA in the concentration range of 1.0-500.0 μg/L.The limits of detection were 0.56,0.26 and 0.53 μg/L,respectively.The recoveries of 6-OH-MTHβC,5-HT and 5-HIAA spiked in rat brain samples were 87.1%-98.2%,87.0%-95.3%,90.1%-97.7%,respectively,and the relative standard deviations of intra-day and inter-day determinations were both less than 6.1%.In comparison with the control,the analysis of alcohol-exposed neonatal rat brain samples revealed a significant difference in the level of 6-OH-MTHβC（P〈0.05）.The method was proved to be simple,highly sensitive,and could be applied in the analysis of 6-OH-MTHβC,5-HT and 5-HIAA in the rat brain.

关 键 词：高效液相色谱 电化学检测 6-羟基-1-甲基-1 2 3 4-四氢-β-咔啉 5-羟色胺 5-羟吲哚乙酸 鼠脑 

分 类 号：O658[理学—分析化学]