4个绿竹地理种源的抗寒性  

Cold resistance in Dendrocalamopsis oldhami from four different geographical provenances

作　　者：黄耀华[1] 郑蓉[2] 邵继锋[1] 方伟[1] 

机构地区：[1]浙江林学院浙江省现代森林培育技术重点实验室,浙江临安311300 [2]福建省林业科学研究院,福建福州350012

出　　处：《浙江林学院学报》2009年第2期188-195,共8页Journal of Zhejiang Forestry College

基　　金：浙江省重大科技攻关项目(2004C12034,2006C12008);浙江省新苗人才计划项目(2007R40G2100022)

摘　　要：为探明4个绿竹Dendrocalamopsis oldhami地理种源的抗寒特性,测定了它们在越冬期间叶片低温半致死温度(LT50)、过氧化物酶(POD)活性、丙二醛(MDA)、脯氨酸和可溶性蛋白质量分数的动态变化。结果表明:越冬过程中,4个绿竹地理种源的LT50值均随温度的下降而下降,并在1月份降到-5.10～-8.14℃;各地理种源绿竹的POD活性、MDA质量摩尔浓度和脯氨酸质量分数均随着气温的升高而增加,但上升幅度因种源而异。各抗寒指标的相关分析表明,可溶性蛋白与POD、MDA和脯氨酸、POD和脯氨酸均呈极显著负相关(P<0.01);纬度与LT50和MDA均呈显著负相关(P<0.05);POD与脯氨酸,LT50与可溶性蛋白均呈极显著正相关(P<0.01)。采用主成分分析法进行抗寒综合评价表明:4个绿竹种源抗寒能力排序为温州种源>福安种源>尤溪种源>漳州种源。To determine cold resistance of Dendrocalamopsis provenances （Wenzhou, Fu＇an, Youxi, and Zhangzhou）, oldhami from four different geographical changes in semilethal temperature （LT50）; peroxidase （POD） activities, malondialdehyde （MDA）, proline and soluble protein content over the course of a winter were compared. Results showed that from the four different geographical provenances the semilethal temperature of Dendrocalamopsis oldhami decreased with the drop in temperature, and decreased to -5.1 to -8.1℃ in January. Over the winter, POD, MDA, and proline content increased with the drop in temperature, but the ranges differed for different geographical provenances. The correlation analysis showed significant, negative correlations between soluble protein in and POD（P〈0.01）, MDA（P〈0.01）, and proline （P〈0.01）; between POD and proline（P〈0.01）; and between latitude and LT50（P〈0.05） and MDA （P〈0.05）. However, there was a highly significant, positive correlation between POD and proline （P〈0.01）, LT50 and soluble protein （P〈0.01）, Principal component analysis indicated that cold-resistance of Wenzhou provenance was best, followed by Fu＇an, Youxi, and Zhangzhou provenances. [Ch, 7 fig. 6 tab. 16 ref.]

关 键 词：植物学 绿竹 半致死温度 丙二醛 抗寒性 种源 

分 类 号：S795.5[农业科学—林木遗传育种] Q948.112[农业科学—林学]