植物体细胞耐盐突变体的研究进展  

Selecting salt-tolerant mutants from somatic plant cells

作　　者：马进[1,2] 鲍沁星[3] 汤庚国[1] 郑钢[2] 

机构地区：[1]南京林业大学风景园林学院,江苏南京210037 [2]浙江林学院园林学院,浙江临安311300 [3]北京林业大学园林学院,北京100083

出　　处：《浙江林学院学报》2009年第2期273-278,共6页Journal of Zhejiang Forestry College

基　　金：浙江省自然科学基金资助项目(Y304072)

摘　　要：植物体细胞耐盐突变体研究已经成为抗盐植物育种学研究的热点问题。综述了植物耐盐体细胞突变体筛选的试材确定、突变体筛选方法和突变体鉴定的研究现状,并指出植物耐盐突变体筛选还存在着变异类型复杂,变异方向难以控制,耐盐细胞系难以再生植株等缺陷。应加强高效稳定的植株再生体系建立;加强细胞悬浮培养技术研究,提高耐盐突变体筛选效率;提高耐盐目标性状的变异频率,减少非目的性状变异产生;从不同层面上加强耐盐突变体基础研究,提高突变体检测效果等方面研究,以提高植物体细胞耐盐突变体育种效果,更好地为实践和生产应用。Selection of salt-tolerant mutants from somatic cells of plants has been an important issue for breeding salt-tolerant plants. This paper introduces current development levels of materials, methods, and identification for the selection of salt-tolerant mutants from somatic cells of plants and points out defects which exist with complex variation types, such as difficulty in controlling the direction of variation and regeneration difficulties with a salt tolerant line of plant cells. Further study should focus on ： 1） establishing a stable and highly efficient plant regeneration system, 2） reinforcing cell suspension culture in order to improve selection efficiency of salt-tolerant mutants from somatic cells of plants, 3） increasing the frequen- cy of variation for salt-tolerant target characteristics to reduce the production of non-target characteristics, and 4） determining a new direction in basic research for detecting salt-tolerant mutants. This would improve salt-tolerant breeding from somatic cells of plants and be useful for application in today＇s world. [ Ch, 36 ref. ]

关 键 词：植物育种学 体细胞 耐盐突变体 筛选 

分 类 号：S754[农业科学—森林经理学]