三维超声微成像监测转基因小鼠前列腺癌模型肿瘤生长  

Three-dimensional ultrasound microimaging for measuring tumor progression in the transgenic mouse adenocarcinoma prostate model

作　　者：武国军[1] 王福利[1] 朱李兵[1] 袁建林[1] 王禾[1] 张更[1] 于磊[1] 杨力军[1] 张运涛[1] 

机构地区：[1]第四军医大学西京医院泌尿外科,陕西西安710032

出　　处：《中国现代医学杂志》2009年第5期658-661,共4页China Journal of Modern Medicine

基　　金：军队医药卫生"十一五"科研项目(No:06H040)

摘　　要：目的应用三维超声微成像监测转基因小鼠前列腺癌(TGMAP)模型的前列腺肿瘤生长。方法使用三维超声微成像系统监测了TGMAP模型小鼠的前列腺肿瘤生长。通过对TGMAP小鼠前列腺癌的三维超声图像和前列腺癌标本的比较,验证该超声系统检测活体小鼠肿瘤大小的可靠性。结果超声成像可检测到直径在2.4～14.0mm的肿瘤。通过三维超声体内测量和尸检获得的肿瘤最大直径的相关性为0.998,超声诊断的敏感性和特异性均>90%。结论小鼠前列腺癌模型的三维超声微成像具有诸多优点,如:空间分辨率高、与软组织的对比强、操作快速简易、设备便宜可携带等,有望成为小鼠临床前期研究的新的微成像手段。[Objective] This article reports the use of three-dimensional ultrasound microimaging for measuring tumor progression in the transgenic mouse adenocarcinoma prostate model （TGMAP）. [Methods] The three-dimensional ultrasound microimaging was used for measuring tumor progression in TGMAP model. Quantitive comparisons of three-dimensional ultrasound images with gross pathology of prostate tumors were done to show the ability of ul- trasound to depict the size of malignant masses in live mice. [Results] Ultrasound imaging identified tumors ranging from 2.4 to 14 mm maximum diameter. The correlation coefficient of tumor diameter measurements done in vivo with three-dimensional ultrasound and at autopsy was 0.998. Prospective tumor detection sensitivity and specificity were both 〉90% when diagnoses were based on repeated ultrasound examinations done on separatedays. [Conclusion] The application of three-dimensional ultrasound imaging to prostate cancer in mice showed advantages, such as high spatial resolution and contrast in soft tissue, fast and uncomplicated protocols, and portable and economical equipment that will likely enable ultrasound to become a new microimaging medality for mouse preclinical trial studies.

关 键 词：前列腺 肿瘤 小鼠模型 三维超声成像 

分 类 号：R392.11[医药卫生—免疫学] R-332[医药卫生—基础医学]