一种用于细胞核成像的新型双光子荧光探针  

New Two-photon Fluorescence Probe for Nuclear DNA Imaging

作　　者：刘鑫[1] 刘恒[1] 贾鹏飞[1] 张波[1] 王军杰[2] 赵宁[2] 张元红[2] 于晓强[2] 

机构地区：[1]兰州大学生命科学学院,兰州730000 [2]山东大学晶体材料国家重点实验室,济南250100

出　　处：《高等学校化学学报》2009年第3期465-467,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:50673053,NSFC/RGC50218001,50173015和30771091)资助

摘　　要：A new two-photon fluorescence DNA probe,BMVEC,with bigger σ×Φ(1437 GM) was synthesized.The absorption titration shows that there is an interaction between BMVEC and DNA because the probe can intercalate the grooves in the double helix of DNA,which enhances its two-photon fluorescent intensity.Meanwhile the double-staining results in cancer cells by means of TPM demonstrate the exclusive nucleic labeling ability of BMVEC,and the positions and amounts of nuclei labeled by BMVEC are wholly the identical as those by DAPI.Moreover,the incident power of BMVEC is lower than that of DAPI.The results indicate that BMVEC can act as the two-photon fluorescent probe of DNA.A new two-photon fluorescence DNA probe, BMVEC, with bigger σ×Ф 1437 GM） was synthesized. The absorption titration shows that there is an interaction between BMVEC and DNA because the probe can intercalate the grooves in the double helix of DNA, which enhances its two-photon fluorescent intensity. Meanwhile the double-staining results in cancer cells by means of TPM demonstrate the exclusive nucleic labe-ling ability of BMVEC, and the positions and amounts of nuclei labeled by BMVEC are wholly the identical as those by DAPI. Moreover, the incident power of BMVEC is lower than that of DAPI. The results indicate that BMVEC can act as the two-photon fluorescent probe of DNA.

关 键 词：双光子荧光探针 双光子荧光成像 核酸 

分 类 号：O657[理学—分析化学]