碳纳米管的快速糖基化及用于糖-凝集素特异性识别作用的研究  

Rapid Glycosylated Functionalization of Single-walled Carbon Nanotubes for Lectin Recognition

作　　者：付群[1] 吴明红[1] 焦正[1] 王德庆[1] 

机构地区：[1]上海大学环境与化学工程学院纳米化学与生物学研究所,上海201800

出　　处：《高等学校化学学报》2009年第3期525-529,共5页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:40830744);上海市曙光计划(批准号:07SG46);新世纪优秀人才计划(批准号:NCET-05-0434)资助

摘　　要：将含糖基的简单两亲分子N-十八烷基麦芽糖酰胺(N-n-Octadecyl-D-maltonamide,NOMA)非共价修饰到单壁碳纳米管(SWNT)表面形成糖-碳纳米管复合体(NOMA-SWNT),谱学和形态学结果表明,NOMA不仅能快速、高效地吸附到SWNT表面,而且能有效地改善SWNT在水溶液中的分散性能.以NOMA-SWNT管束为导通沟道构建了碳纳米管场效应管(CNTFET)器件,检测了麦芽糖和伴刀豆凝集素蛋白(Con A)的特异性识别作用.检测器件在每个修饰阶段的电学性能的变化证明了NOMA对SWNT的非共价糖基化修饰及用CNTFET来检测糖-凝集素特异性识别作用的可能性.Single-walled carbon nanotube （SWNT） was functionalized with N-n-octadecyl-D-maltonamide （ NOMA）, a simple sugar-containing amphipathic compound, by a method of noncovalent adsorption to form a NOMA-SWNT nanocomposite. The spectroscopic and morphology results show that NOMA could be rapidly and effectively adsorbed on the surface of SWNT without changing the native structure of NOMA and the structure properties of SWNT. Moreover, it was shown that the dispersion ability of SWNT in aqueous solution had a significant improvement after coating with NOMA. Field effect transistor（FET） devices with a NOMA-SWNT bundle as the conducting channel have been developed to detect maltose-Concanavalin A（ Con A） interaction. Obvious changes in the Carbon nanotube field effect transistor（CNTFET） characteristics have been detected after NOMA functionalization and after Con A adsorption to the NOMA coating layer, which demenstrates the possobility of noncovalent glycosylated functionalization of SWNTs with simple molecue NOMA and sugar-lectin recognition detection by CNTFET. The presented method for functionalization of SWNT had several advanta- ges, such as rapid and facile CNT functionalization, good hydrophilicity and biocompatibility, versatile detec- tion of receptor via various glycoligand molecule, etc. , and could be used for bio-functionalized materials, de- tection of carbohydrate-protein interactions and diagnose processes of deleterious disease.

关 键 词：单壁碳纳米管 糖基化 糖-碳纳米管复合物 碳纳米管场效应管 糖-凝集素识别 

分 类 号：O647.3[理学—物理化学]