微过氧化物酶-11在壳聚糖修饰玻碳电极上的电化学  

Electrochemistry of Microperoxidase-11(MP-11) on Glassy Carbon Electrode Modified with Chitosan

作　　者：王琨琦[1,2,3] 马中苏[1] 陆天虹[2] 邢巍[2] 朱琳[2] 吕强[1] 邢沈阳[4] 

机构地区：[1]吉林大学生物与农业工程学院,长春130025 [2]中国科学院长春应用化学研究所,长春130022 [3]长春工程学院理学院,长春130021 [4]吉林大学畜牧兽医学院,长春130062

出　　处：《高等学校化学学报》2009年第3期553-556,共4页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:20433060;20876153和20703043)资助

摘　　要：采用物理吸附法,将微过氧化物酶-11(MP-11)固定在载体壳聚糖修饰的玻碳电极表面.运用循环伏安法对MP-11在该修饰电极上的直接电化学行为及对氧(O2)和过氧化氢(H2O2)的电催化行为进行了表征.研究结果表明,在pH=7.12的磷酸盐缓冲溶液中,壳聚糖修饰电极上的MP-11发生了准可逆的氧化还原反应,而且在反应过程中包含质子的传递过程,完全实现了MP-11在该修饰电极上的直接电化学.该修饰电极也可以对O2和H2O2进行电催化还原,并且两个反应的电催化还原过程都是受表面控制的电化学过程,对H2O2催化还原产生的响应电流与H2O2的浓度呈线性关系.In this paper, microperoxidase-ll （MP-11） was immobilized on glassy carbon electrode surface modified with chitosan by physical adsorption. The direct electrochemistry and the electrocatalytic behaviours to O2 and the H2O2 of MP-11 on glassy carbon electrode modified with chitosan were characterized by cyclic vohammetry. The results indicate that MP-11 on modified electrode displays a quasi-reversible electrochemical process coupled with proton transfer in the phosphate buffer solutions（ pH = 7.12）. Direct electrochemical re- action of MP-11 on modified electrode has been realized. MP-11 on modified electrode can catalyze reduction for O2 and H2O2. Both of the catalytic reductions are surface-controlled electrochemical process. The response current of MP-11 on modified electrode to H2O2 is linear with hydrogen peroxide concentration. In a word, the electrode modified by chitosan as a matrix material can be used in the biofuell cell and biosensor in the future.

关 键 词：微过氧化酶-11 壳聚糖 电催化 

分 类 号：O643[理学—物理化学] O614[理学—化学]