超声波联合曲拉通X-100制备猪去细胞的主动脉支架  

Preparation of porcine thoracic aorta acellular tissue scaffold by ultrasound wave combined with TritonX-100

作　　者：张永生[1] 莫绪明[1] 朱建宝[2] 彭卫[1] 何晓敏[1] 束亚琴[1] 

机构地区：[1]南京医科大学附属南京儿童医院胸心外科,210008 [2]东南大学医学院人体解剖学与组织胚胎学系

出　　处：《中华实验外科杂志》2009年第4期494-496,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨超声波联合曲拉通X-100制备猪升主动脉去细胞支架的方法及效果。方法从体质量相近的中华实验猪制备新鲜主动脉标本280份，随机分为4组：空白对照组、曲拉通X-100（Triton X-100）和SDS（十二烷基磺酸钠）组、曲拉通X-100组、超声波联合曲拉通X-100组，通过组织学及电镜观察、生物力学性能测定、免疫组织化学检测Ln和Fn的蛋白表达变化等分析比较各组去细胞的效果。结果超声波联合曲拉通X-100组可完全去除动脉内膜细胞，组织形态、生物力学等保持良好。层粘连蛋白（Ln）和纤维连接蛋白（Fn）的阳性面积×平均吸光度值分别是0．01470±0．00160、0．01340±0．00029，明显优于传统的化学去细胞方法。结论超声波联合曲拉通X-100组去细胞效果优于传统化学去污剂，是较理想的制备猪动脉去细胞支架的方法。Objective To investigate the method for preparation of thoracic aorta acellular tissue scaffold by ultrasound wave combined with TritonX-100. Methods Two hundred and eighty fresh thoracic aorta specimens from China experiment swine with similar body weight were divided into 4 groups：fresh thoracic aorta group, decellularisation group with ultrasound wave and TritonX-100, decellularisation group with SDS and TritonX-100, and decellularisation group with TritonX-100. The effect of decellularisation was compared by histology, scanning electron microscopy, heat shrinkage temperature, mechanics performance. As biomarkers of extracellular matrix,laminin （Ln） and fibronectin （Fn） on the processed tissue specimens were stained with immunohistochemical methods. Results In the acellular scaffold group by ultrasound wave with TritonX-lO0, cells were removed thoroughly, and tissue structure and biomechanics were normal;The average positive staining area of Ln or Fn x the average A value was 0. 0147± 0. 0016 and 0.013 40± 0. 000 29 respectively. Conclusion The method of acellular tissue scaffold by ultrasound wave combined with TritonX-100 is superior to traditional chemical methods.

关 键 词：主动脉 支架 组织工程 超声波 

分 类 号：R318.08[医药卫生—生物医学工程]