人类偏肺病毒感染T细胞亚群和细胞因子的变化及其致喘息作用探讨  

Changes of T-lymphocyte subsets and cytokines in human metapneumo-virus infection and its risk in wheezing

作　　者：廖华[1] 张寿斌[2] 邹红梅[3] 汪小娟[4] 龚鸿裕[3] 钟馥霞[5] 黄艳[1] 林吉涛[1] 

机构地区：[1]深圳市第八人民医院急诊儿科,广东深圳518101 [2]深圳市第八人民医院海富社康中心,广东深圳518101 [3]深圳市第八人民医院急诊科,广东深圳518101 [4]深圳市第八人民医院核医学科,广东深圳518101 [5]深圳市第八人民医院检验科,广东深圳518101

出　　处：《中国儿童保健杂志》2009年第2期162-164,共3页Chinese Journal of Child Health Care

基　　金：深圳市科技计划项目(医疗卫生类)(200603143)

摘　　要：【目的】了解小儿人类偏肺病毒(human metapneumo-virus,hMPV)感染CD4+、CD8+细胞,IL-4、IFN-γ和lgE变化情况,探讨其与喘息性疾病之间关系。【方法】225例下呼吸道感染患儿分别检测hMPV特异性基因,外周血CD4+、CD8+细胞,IL-4、IFN-γ和lgE。【结果】①共检出hMPV29例(12.9%),伴喘息症状者hMPV检出率(15.2%)明显高于不伴喘息症状者(7.5%)(P<0.01);②毛细支气管炎、喘息性支气管炎、哮喘发作组CD4+、CD8+,IFN-γ下降,CD4+/CD8+,IL-4I、gE、IL-4/IFN-γ升高(P<0.05或<0.01);③hMPV(+)和hMPV(-)组同对照组比较,CD4+、CD8+、IFN-γ下降,CD4+/CD8+I、L-4、IgE升高(P<0.05),但两组间比较差异无显著性(P>0.05);④hMPV感染患儿IL-4、IL-4/IFN-γ与IgE呈正相关,IFN-γ与IgE呈负相关(P<0.01)。【结论】hMPV感染患儿T细胞亚群,细胞因子改变明显,免疫功能紊乱,且免疫功能紊乱可能是导致喘息的重要原因之一。[Objective] To investigate the changes of CD4^＋ ,CD8^＋ ,IL-4,IFN-γ and IgE in young children infected by Human Metapneumo-virus（hMPV）, and the relation between hMPV and wheezing. [Methods] The special gene of hMPV and the level of CD4^＋ .CD8^＋ .IL-4.IFN-γ.lgE were checked in 225 LRTIs. [Results] ①Twenty nine（12.9%） were hMPV positive in all, the hMPV rate（ 15.2%） of those with wheeze was higher than those（7.5%）with no wheeze（P〈0.01）. ②The levels of CD4^＋ , CD8^＋ and IFN-γ declined, while The levels of CD4^＋/CD8^＋ , IL-4, IgE and IL-4/IFN-γ rised（P〈0.05 or G0.01）in children with bronchiolitis, wheezing bronchitis or asthma break out.③The levels of CD4^＋ , CD8^＋ and IFN-γ declined, while the levels of CD4^＋/CD8^＋ ,IL-4,IgE and IL-4/IFN-γ rised（P〈0.05 or 〈0.01 ）in both hMPV（ ＋ ） and hMPV（ - ） groups,there was no significant between hMPV（ ＋ ） and hMPV（-） groups （P〈0.05）. ④ The level of IgE was positive relatived with those of IL-4,IL-4/IFN-γ（P〈0.01）,while it was negative relatived with IFN-γ（P〈0.01）. [Conclusions] The levels of T-lymphocyte Subsets （CD4^＋ ,CD8^＋ ） and Cytokines（ IL-4,IFN-γ, IgE） are changed greatly in young children infected by hMPV, and its immunity function is disorded; hMPV infection is an important risk factor in wheezing.

关 键 词：喘息 人类偏肺病毒 T细胞亚群 细胞因子 

分 类 号：R725.6[医药卫生—儿科]