高效液相色谱法测定葛枣弥猴桃叶中黄酮苷A的含量  

Determination of flavonol glycoside A in the leaves of Actinidia polygama(Sieb. et Zucc.) Miq by HPLC

作　　者：姚华[1] 陆娟[1] 刘梦韩[1] 时晓磊[1] 桂明玉[1] 金永日[1] 

机构地区：[1]吉林大学化学学院,吉林长春130012

出　　处：《东北师大学报（自然科学版）》2009年第1期137-140,共4页Journal of Northeast Normal University(Natural Science Edition)

基　　金：吉林省科技发展计划项目(20060903)

摘　　要：采用高效液相色谱法测定了葛枣猕猴桃叶中一种黄酮苷类成分——山奈酚-3-O-α-L-鼠李糖基-（1→3）-α-L-鼠李糖基-（1→6）-β-D-吡喃半乳糖苷（黄酮苷A）的含量.用十八烷基硅烷键合硅胶做填充剂,甲醇-1%乙酸（乙酸体积分数为0.1%）做流动相,检测波长为267nm.结果表明：线性范围为0.099～9.99μg;平均回收率为102.3%;相对标准偏差为1.5%.To establish a quantitative method for determination of one main flavonol glycoside[kaempferol-3-O-α-L-rhamnopyranosyl-（1→3）-α-L-rhamnopyranosyl-（1→6）-β-D-galactopyranoside] in the leaves of Actinidia polygama（Sieb.et Zucc.） Miq.This method was performed on the Eclipse XDB-C18 column with a mobile phase of methanol-water and the detection wavelength was 267 nm.The linearity of this method was good in the range of 0.099～9.99 μg and the average recoveries of A was 102.3%,and the RSD was 1.5%.The method is accurate,reliable and reproducible for determination of A in the leaves of Actinidia polygama.

关 键 词：葛枣猕猴桃 黄酮苷A 反相高效液相色谱法 含量测定 

分 类 号：O657.72[理学—分析化学]