抗旱、耐盐基因P5CS转化向日葵自交系  

Drought and Salt-alkali Resistance Gene P5CS Transformed into Sunflower Inbred Lines

作　　者：程继东[1,2] 安玉麟[1] 孙瑞芬[1] 

机构地区：[1]内蒙古农牧业科学院生物技术中心,呼和浩特010031 [2]内蒙古农业大学农学院,呼和浩特010019

出　　处：《生物技术通报》2009年第3期65-69,共5页Biotechnology Bulletin

基　　金：农业部“948”项目(2006-G23)

摘　　要：以向日葵保持系75-33B和恢复系Ht80-97的子叶为转化受体材料,用携带P5CS基因的双元载体系统的根癌农杆菌进行叶盘转化,获得抗Kan的转化芽。提取转化芽总DNA进行PCR检测及PCR-Southern杂交检测,证实获得阳性抗性芽75-33B 2株,Ht80-97 4株,转化率分别为3.17%和4.65%。The leaf discs of sunflower maintainer fine 75-33B and restorer fine Ht80-97 were transformed with PSCS gene mediated by Agrobacterium tumefaciens which carried a binary vector pCHF3/P5CS. Transgenic shoots were obtained and detected by PCR and PCR-Southem blotting. The results indicated that two transgenic shoots from maintainer line 75-33B and four from restorer line Ht80-97 were positive, and the frequency of genetic transformation was 3.17% and 4.65% respectively.

关 键 词：向日葵 P5CS基因 遗传转化 PCR扩增 PCR—Southern杂交 

分 类 号：S565.5[农业科学—作物学] S792.11