PKC抑制剂对子痫前期血清诱导人脐静脉内皮细胞NF-κB核转位及VCAM-1表达的影响  

Inhibitory effects of polymyxin B on the expression of NF-κB and VCAM-1 in cultured human umbilical vascular endothelial cell induced by serum of preeclampsia

作　　者：蒋荣珍[1] 滕银成[1] 黄亚绢[1] 顾京红[1] 李明[1] 

机构地区：[1]上海交通大学附属第六人民医院妇产科,上海200233

出　　处：《现代妇产科进展》2009年第3期161-164,共4页Progress in Obstetrics and Gynecology

摘　　要：目的：探讨子痫前期血清诱导人脐静脉内皮细胞（HUVEC）蛋白激酶（PKC）活性改变、核因子-κB（NF-κB）核转位及内皮细胞黏附分子-1（VCAM-1）的表达以及PKC抑制剂对它们的影响。方法：用胰酶消化培养法培养正常妊娠HUVEC,传代后待细胞长满至70%~80%后,加或不加PKC抑制剂多黏菌素B（PMB）作用30m in后分别加入正常妊娠及子痫前期血清,培养2h,W estern B lot测定细胞胞浆PKC、胞膜PKC含量、胞浆核因子κB抑制因子（I-κB）、胞核NF-κBp65含量;培养48h,用MTT测定细胞活力,流式细胞学测定细胞凋亡,酶联免疫法测定HUVEC VCAM-1的表达。结果：子痫前期血清培养的HUVEC胞浆PKC及I-κB含量明显低于对照组（P〈0.05）,胞膜PKC含量、胞核NF-κBp65含量、VCAM-1表达、细胞凋亡率明显高于对照组（P〈0.05）,细胞活力明显低于对照组（P〈0.05）。加PKC抑制剂预处理后,子痫前期组HUVEC胞浆PKC含量及I-κB含量明显增加（P〈0.05）;胞膜PKC含量、胞核NF-κBp65含量、VCAM-1表达、细胞凋亡率明显下降（P〈0.05）,细胞活力明显增加（P〈0.05）。结论：子痫前期血清可促进HUVEC NF-κB活性及VCAM-1表达,PKC抑制剂可抑制子痫前期患者血清诱导HUVEC的NF-κB活性及VCAM-1表达,PKC、NF-κB在子痫前期内皮细胞损伤过程中可能起重要的桥梁作用。Objective：To study the effects of polymyxin B on the nuclear factor kappaB（NF-κB） activation,the proliferation and the expression of vascular cell adhesion molecules-1（VCAM-1） of cultured human umbilical vascular endothelial cell（HUVEC） induced by preeclamptic serum.Method：HUVEC were incubated with preeclamptic sera in the presence or in the absence of PMB.The expression of particulate fraction and cytoplasmic protein kinase C（PKC）,cytoplasmic I-κB,nuclear NF-κBp65 were detected by Western blotting after HUVEC was incubated for 2h.The apoptosis of HUVEC were analyzed by flow cytomerty.The expression of VCAM-1 was determined using ELISA after HUVEC was incubated for 48h.Results：Preeclamptic sera could stimulate the apoptosis,the activation of PKC,the activation of NF-κB,and the expression of VCAM-1 of HUVEC as compared with normal umbilical sera（P〈0.05）.PMB could inhibit the apoptosis,the activation of PKC,the activation of NF-κB,and the expression of VCAM-1 of HUVEC stimulated by preeclamptic sera（P〈0.05）.Conclusion：PMB can inhibit the activation of NF-κB and the expression of VCAM-1 of HUVEC stimulated by preeclamptic sera,PKC-NF-κB signal transduction pathway may play a key role in the injury of endothelial cell in preeclampsia.

关 键 词：蛋白激酶C 核因子-κB 脐静脉内皮细胞 多黏菌素B 内皮细胞黏附分子-1 

分 类 号：R714.24[医药卫生—妇产科学]