TGF—β1对新生缺氧缺血性脑损伤大鼠的影响  

Brain protection of TGF-β1 on newborn rats with hypoxic-ischemic brain damage

作　　者：李小权[1] 孙晓勉 杨周岐[1] 王丽[3] 

机构地区：[1]西安交通大学医学院第一附属医院儿科,陕西西安710061 [2]深圳市福田区妇幼保健院儿保科,广州深圳518020 [3]西安市北方医院急诊科,陕西西安710032

出　　处：《中国妇幼健康研究》2009年第2期143-145,共3页Chinese Journal of Woman and Child Health Research

基　　金：[基金项目]陕西省科技攻关资助项目（20006K14-G12）

摘　　要：目的探讨TGF-β1对新生大鼠缺氧缺血性脑损伤模型的保护作用。方法95只新生不分性别的7日龄SD大鼠随机分为正常对照组、假手术组、模型组、干预组（盐水组及治疗组），模型建立成功后2小时，干预组大鼠腹腔注入10ng的盐水或TGF-β1，用免疫组织化学方法观察建立模型后2小时及给药后24小时脑组织海马CA1区锥体细胞凋亡的情况和Caspase-3表达的变化。结果建模后2小时模型组与正常对照组和假手术组比较，脑海马CA1区锥体细胞凋亡明显增加（U1=11．58、U2=11．50．均P〈0．01），Caspase-3表达增强（U1=3．49、U2=5．31，均P〈0．01）；给药后24小时模型纽与正常对照组和假手术组比较，脑海马CA1区锥体细胞凋亡明显增强（U1=14．56、U2=14．65，均P〈0．01），Caspase-3表达增强（U1=4．95、U2=5．91，均P〈0．01）；治疗组与模型组和盐水干预组比较，脑海马CA1区锥体细胞凋亡明显减轻（U1=11．39、U2=9．19，均P〈0．01），Caspase-3表达减少（U1=3．34、U2=2．83，均P〈0．01）。结论脑缺氧缺血后应用TGF-β1具有明显的保护作用，TGF-β1可显著减少新生大鼠脑缺氧缺血后海马CA1区锥体细胞的凋亡，TGF-β1保护神经元、减轻凋亡可能是通过减少凋亡相关蛋白Caspase-3的表达起作用的。Objective To investigate brain protection of transform growth factor β1 （TGF-β1） in a newborn rat model of hypoxic-ischemic brain damage （HIBD）. Methods 95 unsexed newborn SD rats aged 7 days were randomly divided into four groups： normal control （NC） group, pseudosurgical group, hypoxic-ischemic brain damage （HIBD） model group and intervention group （normal saline intervention subgroup and TGF-β1 treatment subgroup）. At 2 hours after establishment of HIBD model, the rats in the intervention group were injected 10ng of normal saline or TGF-β1 in their abdominal cavity. The apoptosis of pyramidal cells in hippocampus CA1 region of rats and expression of Caspase-3 at 2 hours after establishment of the model and 24 hours after administration in the HIBD model group were detected with immunohistochemical method. Results As compared with NC group and pseudosurgery group, at 2h after establishment of HIBD model, the apoptosis of pyramidal ceils in hippocampus CA1 region of rats in the HIBD model group was significantly increased （ U1 = 11.58, U2 = 11.50,both P 〈 0.01 ） and expression of Caspase-3 was up-regulated （ U 1= 3.49, U2 = 5.31,both P 〈 0.01 ）. At 24 hours after administration, the apoptosis of pyramidal cells in hippocampus CA1 region of rats in the HIBD model group was still significantly increased （ U1 = 14.56, U2 = 14.65, both P 〈 0. 01 ） and the expression of Caspase-3 kept up-regulated （ U1 = 4.95, U2 = 5.91 ,both P 〈 0.01 ） as compared with NC group and pseudosurgery group. While the apoptosis of pyramidal cells in hippocampus CA1 region of rats in cytokine TGF-β1 treatment subgroup was significantly decreased as compared with the model group and normal saline intervention group （ U1 = 11.39, U2 = 9.19, both P 〈 0.01 ） and the expression of Caspase-3 was also decreased （ U1 = 3.34, U2 = 2.83, both P 〈 0.01 ）. Conclusion TGF-β1 can significantly decrease apoptosis of pyramidal cells in hippocampus CA1 region of the newborn rats after hypoxia-

关 键 词：转化生长因子Β1 缺氧缺血 脑 新生SD大鼠 

分 类 号：R722[医药卫生—儿科]