大鼠肝再生早期枯否细胞介导的Caspase-3激活机制探讨  

Role of kupffer cell in activation of Caspase-3 during the early stage of liver regeneration in rats

作　　者：时多[1,2] 周运恒[3] 张颖玮[3] 袁斌[1] 袁星[1] 焦炳华[1] 缪明永[1] 

机构地区：[1]第二军医大学基础部,上海200433 [2]第二军医大学药学系 [3]第二军医大学长征医院

出　　处：《山东医药》2009年第10期7-9,共3页Shandong Medical Journal

摘　　要：目的探讨大鼠肝再生早期枯否细胞介导的肝细胞凋亡执行酶Caspase-3的激活机制。方法制备肝再生模型,部分用肝枯否细胞的特异性抑制剂氯化钆(Gd)处理。计算肝再生度和再生指数,检测血清ALT、AST和TNF-α,检测肝组织Caspase-3、8活性。结果与未经Gd处理的模型组相比,Gd处理模型72h时肝再生度和再生指数降低,24h时血清ALT及AST水平升高,而TNF-α水平下降,3h和6h时肝组织Caspase-3、8活性下降。结论肝再生早期,枯否细胞通过Caspase-8等介导的外源性凋亡信号途径参与Caspase-3的激活。Objective To analyze the activation mechanism of hepatic apoptotic enzyme Caspase-3 during the early stage of liver regeneration from the perspective of Kupffer cell. Methods Liver regeneration models were finished and partly were administrated by gadolinium chloride （ Gd ）, liver regeneration index （ LRI ） and liver regeneration degree （ LRD ） were observed. Serum AST,ALT and TNF-α, serum Caspase-3 and 8 activities were determined. Results Compared with control group, Gd treatment decreased LRI and LRD at 72 h, increased serum AST and ALT levels at 24 h, decreased serum TNF-α level at 24 h, and decreased activities of Caspase-3 and 8 at 3 h and 6 h. Conclusion During the early stage of liver regeneration,Kupffer cell may contribute to activation of apoptotie enzyme Caspase-3 through signal pathway mediated by caspase-8 and other signal molecules.

关 键 词：肝脏再生 细胞凋亡 凋亡执行酶 枯否细胞 

分 类 号：R657.3[医药卫生—外科学]