缝隙连接蛋白和肠间质细胞在HD患儿结肠壁的分布  

Localization of gap juction and interstitial cells of Cajal in colon of patients with Hirschsprung's disease

作　　者：何智丽[1] 陈琦[1] 曹振杰[1] 

机构地区：[1]郑州大学第三附属医院,河南郑州450052

出　　处：《山东医药》2009年第10期14-15,共2页Shandong Medical Journal

摘　　要：目的观察缝隙连接蛋白43(Cx43)及肠间质细胞(ICC)在先天性巨结肠(HD)患儿结肠壁的分布情况。方法取30例HD患儿扩张段及狭窄段肠管标本、15例正常结肠组织,行免疫组化检查,并取5例HD患儿新鲜结肠标本在透射电镜下观察。结果HD狭窄段肠管壁内Cx43及ICC均明显减少,与HD扩张段肠壁和正常结肠相比,P均<0.05。电镜下狭窄段肠管偶见缝隙连接,且稀疏,扩张段平滑肌及ICC之间可见到缝隙连接。结论HD狭窄段肠管壁内Cx43表达及ICC数目减少,是HD肠动力功能障碍的重要原因之一。Objective To investigate the localization of gap juction protein 43 （ Cx43 ） and interstitial cells of Cajal （ICC） in Hirschspnmg＇s disease（HD） colon. Methods 30 cases with HD and 15 cases of nomal colon were studied by immunohistocbemical. 5 fresh colon segments of HD were detected by transmission electron microscopy. Results There was no or weak of Cx43 and ICC in the colon wall of aganglionic part of HD. There was significant different between aganglionic part and ganglionic part or nomal colon,P 〈0.05. Gap junction was less in the aganglionic part of HD,but it could be observed more in ganglionic part of HD. Conclusion The lack of Cx43 and ICC in the aganglionic bowel of HD may partly be responsible for the motility dysfunction in HD.

关 键 词：先天性巨结肠 缝隙连接 肠间质细胞 

分 类 号：R726.2[医药卫生—儿科]